| dc.creator | Nogueira, Raquel Tayar | |
| dc.creator | Nogueira, Alanderson Rocha | |
| dc.creator | Pereira, Mirian Claudia Souza | |
| dc.creator | Rodrigues, Maurício Martins | |
| dc.creator | Neves, Patrícia Cristina da Costa | |
| dc.creator | Galler, Ricardo | |
| dc.creator | Bonaldo, Myrna Cristina | |
| dc.date | 2015-08-19T13:49:34Z | |
| dc.date | 2015-08-19T13:49:34Z | |
| dc.date | 2013 | |
| dc.date.accessioned | 2023-09-26T23:20:46Z | |
| dc.date.available | 2023-09-26T23:20:46Z | |
| dc.identifier | NOGUEIRA, Raquel Tayar; et al. Recombinant Yellow Fever Viruses Elicit CD8+ T Cell Responses and Protective Immunity against Trypanosoma cruzi. Plos One, v8, n.3, 13p, 2013. | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/11568 | |
| dc.identifier | 10.1371/journal.pone.0059347 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8889561 | |
| dc.description | Chagas’ disease is a major public health problem affecting nearly 10 million in Latin America. Despite several experimental
vaccines have shown to be immunogenic and protective in mouse models, there is not a current vaccine being licensed for
humans or in clinical trial against T. cruzi infection. Towards this goal, we used the backbone of Yellow Fever (YF) 17D virus,
one of the most effective and well-established human vaccines, to express an immunogenic fragment derived from T. cruzi
Amastigote Surface Protein 2 (ASP-2). The cDNA sequence of an ASP-2 fragment was inserted between E and NS1 genes of
YF 17D virus through the construction of a recombinant heterologous cassette. The replication ability and genetic stability
of recombinant YF virus (YF17D/ENS1/Tc) was confirmed for at least six passages in Vero cells. Immunogenicity studies
showed that YF17D/ENS1/Tc virus elicited neutralizing antibodies and gamma interferon (IFN-c) producing-cells against the
YF virus. Also, it was able to prime a CD8+ T cell directed against the transgenic T. cruzi epitope (TEWETGQI) which expanded
significantly as measured by T cell-specific production of IFN-c before and after T. cruzi challenge. However, most important
for the purposes of vaccine development was the fact that a more efficient protective response could be seen in mice
challenged after vaccination with the YF viral formulation consisting of YF17D/ENS1/Tc and a YF17D recombinant virus
expressing the TEWETGQI epitope at the NS2B-3 junction. The superior protective immunity observed might be due to an
earlier priming of epitope-specific IFN-c-producing T CD8+ cells induced by vaccination with this viral formulation. Our
results suggest that the use of viral formulations consisting of a mixture of recombinant YF 17D viruses may be a promising
strategy to elicit protective immune responses against pathogens, in general. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Plos One | |
| dc.rights | open access | |
| dc.subject | Trypanosoma cruzi | |
| dc.subject | Chagas Disease | |
| dc.subject | Yellow Fever (YF) 17D virus | |
| dc.subject | Trypanosoma cruzi | |
| dc.subject | Doença de Chagas | |
| dc.subject | Vírus da Febre Amarela | |
| dc.title | Recombinant Yellow Fever Viruses Elicit CD8+ T Cell Responses and Protective Immunity against Trypanosoma cruzi | |
| dc.type | Article | |
