Biological study of Trypanosoma caninum under co-culture with different feeder layer cells

dc.creatorFonseca-Oliveira, Tatiana S.
dc.creatorBarros, Juliana Helena S.
dc.creatorMadeira, Juliana Bernardo
dc.creatorPacheco, Raquel da Silva
dc.creatorAlves, Carlos Roberto
dc.creatorCôrtes, Luzia M. C.
dc.creatorMarzochi, Mauro Célio de A.
dc.creatorMadeira, Maria de Fatima
dc.date2019-02-15T12:45:09Z
dc.date2019-02-15T12:45:09Z
dc.date2018
dc.date.accessioned2023-09-26T23:20:26Z
dc.date.available2023-09-26T23:20:26Z
dc.identifierFONSECA-OLIVEIRA, Tatiana S. et al. Biological study of Trypanosoma caninum under co-culture with different feeder layer cells. Acta Tropica, v. 87, p. 44-50, 2018.
dc.identifier0001-706X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31669
dc.identifier10.1016/j.actatropica.2018.07.011
dc.identifier1873-6254
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8889499
dc.descriptionTrypanosoma caninum is a parasite isolated from domestic dogs, of which several biological aspects remain unknown, including evolutive forms found in vertebrate hosts. The objective of this study was to evaluate co-cultures of T. caninum with different cell lines as feeder layers to monitor the differentiation process and investigate infective potential. The study was performed using DH-82, MDCK, and Lulo cell lines. T. caninum from axenic culture was added to the cultured adherent cells. At intervals over 30 days, aliquots of the supernatant were collected for quantification and assessment of differentiation. Infectivity assays were performed on the aforementioned cell lines seeded on glass coverslips and evaluated after 6, 24, and 72 h. In the supernatant of the feeder layer, T. caninum presented similar growth profiles, with epimastigote and trypomastigote forms in binary and multiple divisions. During co-culture with DH-82 and MDCK cells, a higher level of differentiation to trypomastigotes was observed. This study shows that the differentiation process of this parasite can vary according to culture conditions and that DH-82 and MDCK lineages could be applied to the study of trypomastigote forms. All forms of T. caninum described until now (aflagellar epimastigotes, typical epimastigotes, or trypomastigotes) were unable to infect the cell line Finally, this study provides additional data about morphobiological aspects. Although the biological cycle of T. caninum has not been established, the present data suggest the importance of feeder layers in promoting the growth and differentiation of this new parasite.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectTrypanosoma caninum
dc.subjectCell lines
dc.subjectEvolutive forms
dc.titleBiological study of Trypanosoma caninum under co-culture with different feeder layer cells
dc.typeArticle


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