Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach

dc.creatorIñiguez, Alena Mayo
dc.creatorAraújo, Adauto José Goncalves de
dc.creatorSilva, Luiz Fernando Rocha Ferreira da
dc.creatorVicente, Ana Carolina Paulo
dc.date2019-08-19T12:58:56Z
dc.date2019-08-19T12:58:56Z
dc.date2003
dc.date.accessioned2023-09-26T23:20:24Z
dc.date.available2023-09-26T23:20:24Z
dc.identifierIÑIGUEZ, Alena M et al. Analysis of ancient DNA from coprolites: a perspective with random amplified polymorphic DNA-polymerase chain reaction approach. Memórias do Instituto Oswaldo Cruz, v. 98, suplem. 1, p. 1-3, 2003.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/34869
dc.identifier10.1590/S0074-02762003000900012
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8889491
dc.descriptionThe aim of this work was to determine approaches that would improve the quality of ancient DNA (aDNA) present in coprolites to enhance the possibility of success in retrieving specific sequence targets. We worked with coprolites from South American archaeological sites in Brazil and Chile dating up to 7,000 years ago. Using established protocols for aDNA extraction we obtained samples showing high degradation as usually happens with this kind of material. The reconstructive polymerization pretreatment was essential to overcome the DNA degradation and the serial dilutions helped with to prevent polymerase chain reaction (PCR) inhibitors. Moreover, the random amplified polymorphic DNA-PCR has been shown to be a reliable technique for further experiments to recover specific aDNA sequences.
dc.formatapplication/pdf
dc.languageeng
dc.publisherInstituto Oswaldo Cruz
dc.rightsopen access
dc.subjectMolecular Paleoparasitology
dc.subjectCoprolites
dc.subjectRandom Amplified Polymorphic DNA
dc.subjectAncient DNA
dc.subjectPolymerase Chain Reaction
dc.titleAnalysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach
dc.typeArticle


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