| dc.creator | Bourgard, Catarina | |
| dc.creator | Lopes, Stefanie C. P. | |
| dc.creator | Lacerda, Marcus V. G. | |
| dc.creator | Albrecht, Letusa | |
| dc.creator | Costa, Fabio T. M. | |
| dc.date | 2023-02-07T14:18:41Z | |
| dc.date | 2023-02-07T14:18:41Z | |
| dc.date | 2021 | |
| dc.date.accessioned | 2023-09-26T23:20:16Z | |
| dc.date.available | 2023-09-26T23:20:16Z | |
| dc.identifier | BOURGARD, Catarina et al. A suitable RNA preparation methodology for whole transcriptome shotgun sequencing harvested from Plasmodium vivax‑infected patients. Scientific Reports, [s. l.], v. 11, n. 5089, p. 1-12, 2021. | |
| dc.identifier | 2045-2322 | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/56919 | |
| dc.identifier | 10.1038/s41598-021-84607-w | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8889466 | |
| dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) e Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). | |
| dc.description | Plasmodium vivax is a world-threatening human malaria parasite, whose biology remains elusive. The unavailability of in vitro culture, and the difficulties in getting a high number of pure parasites makes RNA isolation in quantity and quality a challenge. Here, a methodological outline for RNA-seq from P. vivax isolates with low parasitemia is presented, combining parasite maturation and enrichment with efficient RNA extraction, yielding ~ 100 pg.µL−1 of RNA, suitable for SMART-Seq Ultra-Low Input RNA library and Illumina sequencing. Unbiased coding transcriptome of ~ 4 M reads was achieved for four patient isolates with ~ 51% of transcripts mapped to the P. vivax P01 reference genome, presenting heterogeneous profiles of expression among individual isolates. Amongst the most transcribed genes in all isolates, a parasite-staged mixed repertoire of conserved parasite metabolic, membrane and exported proteins was observed. Still, a quarter of transcribed genes remain functionally uncharacterized. In parallel, a P. falciparum Brazilian isolate was also analyzed and 57% of its transcripts mapped against IT genome. Comparison of transcriptomes of the two species revealed a common trophozoite-staged expression profile, with several homologous genes being expressed. Collectively, these results will positively impact vivax research improving knowledge of P. vivax biology. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.rights | open access | |
| dc.subject | Plasmodium vivax | |
| dc.subject | Malaria | |
| dc.title | A suitable RNA preparation methodology for whole transcriptome shotgun sequencing harvested from Plasmodium vivax‑infected patients | |
| dc.type | Article | |
