Ehrlichiosis in anemic, thrombocytopenic, or tick-infested dogs from a hospital population in South Brazil

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorDagnone, Ana Silvia
dc.creatorDe Morais, Helio S. Autran
dc.creatorVidotto, Marilda C.
dc.creatorJojima, Flavio S.
dc.creatorVidotto, Odilon
dc.date2014-05-27T11:20:56Z
dc.date2016-10-25T18:19:01Z
dc.date2014-05-27T11:20:56Z
dc.date2016-10-25T18:19:01Z
dc.date2003-11-28
dc.date.accessioned2017-04-06T01:07:09Z
dc.date.available2017-04-06T01:07:09Z
dc.identifierVeterinary Parasitology, v. 117, n. 4, p. 285-290, 2003.
dc.identifier0304-4017
dc.identifierhttp://hdl.handle.net/11449/67478
dc.identifierhttp://acervodigital.unesp.br/handle/11449/67478
dc.identifier10.1016/j.vetpar.2003.10.001
dc.identifier2-s2.0-0345448338
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2003.10.001
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/888918
dc.descriptionEhrlichia canis has a worldwide distribution, but clinical manifestations may vary geographically. We selected 129 dogs to determine prevalence of ehrlichiosis in dogs with anemia, thrombocytopenia, or ticks presented to a Veterinary Teaching Hospital in South Brazil. Of the 129 dogs, 68 carried the brown dog tick (Rhipicephalus sanguineus), 61 had thrombocytopenia (platelet count <150,000/μl), and 19 had anemia (PCV < 22%). Twenty dogs fulfilled more than one inclusion criteria. Ehrlichiosis was diagnosed by positive amplification of ehrlichial DNA by PCR using primers ECC and ECB that amplify a sequence of the 16S rRNA gene. Presence of E. canis was confirmed by cleavage of the amplified DNA using endonucleases HaeIII and AvaI. Fourteen of 68 (21%) dogs with ticks had ehrlichiosis, whereas 12 of 61 (20%) dogs presented with thrombocytopenia and 4 of 19 (21%) anemic dogs had ehrlichiosis. Similar results were obtained in dogs with thrombocytopenia and anemia (one of eight positive) and in dogs with thrombocytopenia and ticks (two of seven positive). All four dogs with anemia and ticks, and the dog that fulfilled all inclusion criteria yield no amplification of ehrlichial DNA by PCR. Based on our results, one in each five dogs infested by the brown dog tick, with anemia or thrombocytopenia had ehrlichosis. Contrary to widespread believe, ehrlichiosis was not the main cause for thrombocytopenia in our region. © 2003 Elsevier B.V. All rights reserved.
dc.languageeng
dc.relationVeterinary Parasitology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAnemia
dc.subjectEhrlichia canis
dc.subjectRhipicephalus sanguineus
dc.subjectThrombocytopenia
dc.subjectbacterial DNA
dc.subjectbacterial enzyme
dc.subjectDNA 16S
dc.subjectendonuclease
dc.subjectribosome RNA
dc.subjectanemia
dc.subjectanimal
dc.subjectanimal disease
dc.subjectBrazil
dc.subjectDNA cleavage
dc.subjectdog
dc.subjectdog disease
dc.subjectehrlichiosis
dc.subjectgene amplification
dc.subjectgene sequence
dc.subjectinfestation
dc.subjectisolation and purification
dc.subjectmicrobiology
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectprevalence
dc.subjectthrombocyte count
dc.subjectthrombocytopenia
dc.subjecttick
dc.subjectAnimals
dc.subjectDNA, Bacterial
dc.subjectDog Diseases
dc.subjectDogs
dc.subjectEhrlichiosis
dc.subjectPrevalence
dc.subjectTick Infestations
dc.subjectTicks
dc.subjectAcari
dc.subjectCanis
dc.subjectCanis familiaris
dc.subjectEhrlichia
dc.subjectRhipicephalus
dc.titleEhrlichiosis in anemic, thrombocytopenic, or tick-infested dogs from a hospital population in South Brazil
dc.typeOtro


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