Structural, cytochemical, immunocytochemical and ultrastructural characterization of blood granulocytes of the roadside hawk Buteo magnirostris (Gmelin, 1788) (Avian, Falconiform)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSantos, A. A.
dc.creatorSilva, A. Marques Joppert da
dc.creatorRegis Silva, M. R.
dc.creatorCômodo Segreto, H. R.
dc.creatorEgami, M. Imoto
dc.date2014-05-27T11:20:55Z
dc.date2016-10-25T18:18:56Z
dc.date2014-05-27T11:20:55Z
dc.date2016-10-25T18:18:56Z
dc.date2003-10-01
dc.date.accessioned2017-04-06T01:06:48Z
dc.date.available2017-04-06T01:06:48Z
dc.identifierJournal of Submicroscopic Cytology and Pathology, v. 35, n. 4, p. 351-357, 2003.
dc.identifier1122-9497
dc.identifierhttp://hdl.handle.net/11449/67437
dc.identifierhttp://acervodigital.unesp.br/handle/11449/67437
dc.identifier2-s2.0-2442651586
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/15137677
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/888882
dc.descriptionIn the peripheral blood of the roadside hawk, Buteo magnirostris, the following types of granulocytic leucocytes were identified: heterophil, eosinophil and basophil. The heterophils presented acidophilic and spindle shaped granules, the eosinophils possess spherical eosinophilic granules and the basophils showed spherical and basophilic granules. The heterophils and eosinophils presented positive cytochemical reaction to glycogen and basic polyaminoacid, while the eosinophils presented sudanophilic granules, which were also positive for myeloperoxidase. The heterophils, alone, presented positivity for acid phosphatase in some granules and immunoreactivity to TGF-β1 was observed only in the cytoplasm of the eosinophils. Electron microscopy demonstrated the heterophil granules as predominantly spindle shaped, being strongly electron-dense, while the eosinophils had numerous uniformly electron-dense spherical granules and the basophils presented three different types of granules identified according to their electron-density and the aspect of their matrix.
dc.languageeng
dc.relationJournal of Submicroscopic Cytology and Pathology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAvian
dc.subjectCytochemistry
dc.subjectGranulocyte
dc.subjectStructure
dc.subjectTGFβ
dc.subjectUltrastructure
dc.subjectacid phosphatase
dc.subjectcancer growth factor
dc.subjectglycogen
dc.subjectheterophile antibody
dc.subjectmyeloperoxidase
dc.subjectpolyaminoacid
dc.subjecttumor growth factor beta1
dc.subjectunclassified drug
dc.subjectanimal cell
dc.subjectbasophil
dc.subjectbird
dc.subjectblood
dc.subjectbuteo magnirostis
dc.subjectcell density
dc.subjectcontrolled study
dc.subjectcytochemistry
dc.subjectcytoplasm
dc.subjectelectron microscopy
dc.subjecteosinophil
dc.subjectgranulocyte
dc.subjecthawk
dc.subjectimmunocytochemistry
dc.subjectimmunoreactivity
dc.subjectleukocyte
dc.subjectnonhuman
dc.subjectreaction analysis
dc.subjectstructure analysis
dc.subjectultrastructure
dc.subjectAnimals
dc.subjectBasophils
dc.subjectEosinophils
dc.subjectGranulocytes
dc.subjectImmunohistochemistry
dc.subjectMicroscopy, Electron
dc.subjectRaptors
dc.subjectTransforming Growth Factor beta
dc.subjectAccipitrinae
dc.subjectAnimalia
dc.subjectAves
dc.subjectButeo
dc.subjectButeo magnirostris
dc.subjectFalconiformes
dc.titleStructural, cytochemical, immunocytochemical and ultrastructural characterization of blood granulocytes of the roadside hawk Buteo magnirostris (Gmelin, 1788) (Avian, Falconiform)
dc.typeOtro


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