Binding of Sulpiride to Seric Albumins

dc.creatorFragoso, Viviane Muniz da Silva
dc.creatorCoura, Carla Patrícia de Morais
dc.creatorHoppe, Luanda Yanaan
dc.creatorSoares, Marília Amável Gomes
dc.creatorSilva, Dilson
dc.creatorCortez, Celia Martins
dc.date2017-02-16T14:50:57Z
dc.date2017-02-16T14:50:57Z
dc.date2016
dc.date.accessioned2023-09-26T23:15:48Z
dc.date.available2023-09-26T23:15:48Z
dc.identifierFRAGOSO, Viviane Muniz da Silva; et al. Binding of Sulpiride to Seric Albumins. Int. J. Mol. Sci., v.17, n.1, 10p, 2016.
dc.identifier1661-6596
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/17838
dc.identifier10.3390/ijms17010059
dc.identifier1422-0067
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8888633
dc.descriptionThe aim of this work was to study the interaction of sulpiride with human serum albumin (HSA) and bovine serum albumin (BSA) through the fluorescence quenching technique. As sulpiride molecules emit fluorescence, we have developed a simple mathematical model to discriminate the quencher fluorescence from the albumin fluorescence in the solution where they interact. Sulpiride is an antipsychotic used in the treatment of several psychiatric disorders. We selectively excited the fluorescence of tryptophan residues with 290 nm wavelength and observed the quenching by titrating HSA and BSA solutions with sulpiride. Stern-Volmer graphs were plotted and quenching constants were estimated. Results showed that sulpiride form complexes with both albumins. Estimated association constants for the interaction sulpiride-HSA were 2.20 (±0.08) × 10⁴ M(-1), at 37 °C, and 5.46 (±0.20) × 10⁴ M(-1), at 25 °C. Those for the interaction sulpiride-BSA are 0.44 (±0.01) × 10⁴ M(-1), at 37 °C and 2.17 (±0.04) × 10⁴ M(-1), at 25 °C. The quenching intensity of BSA, which contains two tryptophan residues in the peptide chain, was found to be higher than that of HSA, what suggests that the primary binding site for sulpiride in albumin should be located next to the sub domain IB of the protein structure.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectAlbuminas
dc.subjectExtinção de fluorescência
dc.subjectInteração
dc.subjectSulpirida
dc.subjectalbumins
dc.subjectfluorescence quenching
dc.subjectinteraction
dc.subjectsulpiride
dc.titleBinding of Sulpiride to Seric Albumins
dc.typeArticle


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