The haemoculture of Trypanosoma minasense chagas, 1908

dc.creatorZiccardi, Mariangela
dc.creatorOliveira, Ricardo Lourenço de
dc.creatorNogueira, Ricardo
dc.date2020-10-05T14:52:20Z
dc.date2020-10-05T14:52:20Z
dc.date1996
dc.date.accessioned2023-09-26T23:14:22Z
dc.date.available2023-09-26T23:14:22Z
dc.identifierZICCARDI, Mariangela; OLIVEIRA, Ricardo Lourenço de; NOGUEIRA, Ricardo. The haemoculture of Trypanosoma minasense chagas, 1908. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 91, n. 4, p. 501-505, July/Aug. 1996.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/43807
dc.identifier10.1590/S0074-02761996000400019
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8888382
dc.descriptionTrypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20% foetal bovine serum, on Novy, McNeal and Nicolle medium (NNN), at 27°C, with a peak around 168 hr. The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacycliclike forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectTrypanosoma minasense
dc.subjectPrimatas neotropicais
dc.subjectTripanossomas
dc.subjectSaguis
dc.subjectFlagelados de Typanosomatidae
dc.subjectCultura
dc.subjectBrasil
dc.subjectTrypanosoma minasense
dc.subjectNeotropical primates
dc.subjectTrypanosomes
dc.subjectMarmosets
dc.subjectTypanosomatidae flagellates
dc.subjectCulture
dc.subjectBrazil
dc.titleThe haemoculture of Trypanosoma minasense chagas, 1908
dc.typeArticle


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