Characterization of the early inflammatory infiltrate at the feeding site of infected sand flies in mice protected from vector-transmitted Leishmania major by exposure to uninfected bites.

dc.creatorTeixeira, Clarissa Romero
dc.creatorGomes, Regis Bernardo Brandim
dc.creatorOliveira, Fabiano
dc.creatorMeneses, Claudio
dc.creatorGilmore, Dana C
dc.creatorElnaiem, Dia-Eldin A
dc.creatorValenzuela, Jesus G
dc.creatorKamhawi, Shaden
dc.date2014-06-03T18:08:48Z
dc.date2014-06-03T18:08:48Z
dc.date2014
dc.date.accessioned2023-09-26T23:14:21Z
dc.date.available2023-09-26T23:14:21Z
dc.identifierTEIXEIRA, C. et al. Characterization of the early inflammatory infiltrate at the feeding site of infected sand flies in mice protected from vector-transmitted Leishmania major by exposure to uninfected bites. PLoS Neglected Tropical Diseases, v. 8, n. 4, p. e2781, 2014.
dc.identifier1935-2735
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7759
dc.identifier10.1371/journal.pntd.0002781
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8888378
dc.descriptionBACKGROUND: Mice exposed to sand fly saliva are protected against vector-transmitted Leishmania major. Although protection has been related to IFN-γ producing T cells, the early inflammatory response orchestrating this outcome has not been defined. METHODOLOGY/PRINCIPAL FINDINGS: Mice exposed to uninfected P. duboscqi bites and naïve mice were challenged with L. major-infected flies to characterize their early immune response at the bite site. Mostly, chemokine and cytokine transcript expression post-infected bites was amplified in exposed compared to naïve mice. In exposed mice, induced chemokines were mostly involved in leukocyte recruitment and T cell and NK cell activation; IL-4 was expressed at 6 h followed by IFN-γ and iNOS2 as well as IL-5 and IL-10 expression. In naïve animals, the transcript expression following Leishmania-infected sand fly bites was suppressed. Expression profiles translated to an earlier and significantly larger recruitment of leukocytes including neutrophils, macrophages, Gr+ monocytes, NK cells and CD4+ T cells to the bite site of exposed compared to naïve mice post-infected bites. Additionally, up to 48 hours post-infected bites the number of IFN-γ-producing CD4+ T cells and NK cells arriving at the bite site was significantly higher in exposed compared to naïve mice. Thereafter, NK cells become cytolytic and persist at the bite site up to a week post-bite. CONCLUSION/SIGNIFICANCE: The quiet environment induced by a Leishmania-infected sand fly bite in naïve mice was significantly altered in animals previously exposed to saliva of uninfected flies. We propose that the enhanced recruitment of Gr+ monocytes, NK cells and CD4 Th1 cells observed at the bite site of exposed mice creates an inhospitable environment that counters the establishment of L. major infection
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectLeishmania Major
dc.subjectLeishmaniose
dc.subjectSaliva
dc.subjectAnimais
dc.subjectRatos
dc.subjectCamundongos
dc.subjectQuimiocinas
dc.subjectInfecção
dc.titleCharacterization of the early inflammatory infiltrate at the feeding site of infected sand flies in mice protected from vector-transmitted Leishmania major by exposure to uninfected bites.
dc.typeArticle


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