Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2

dc.creatorMonteiro, Andrea de Souza
dc.creatorDomingues, Vitor Souza
dc.creatorSouza, Marcus Vinícius Dias
dc.creatorLula, Ivana
dc.creatorGonçalves, Daniel Bonoto
dc.creatorSiqueira Filho, Ezequias Pessoa de
dc.date2014-06-05T13:53:39Z
dc.date2014-06-05T13:53:39Z
dc.date2012
dc.date.accessioned2023-09-26T23:13:58Z
dc.date.available2023-09-26T23:13:58Z
dc.identifierMONTEIRO, Andrea de Souza et al. Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2. Biotechnology for Biofuels. 2012, vol.5, pp. 29.
dc.identifier754-6834
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7798
dc.identifier10.1186/1754-6834-5-29
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8888304
dc.descriptionBackground The microbial bioemulsifiers was surface active compounds, are more effective in stabilizing oil-in-water emulsions. The yeasts have been isolated to produce bioemulsifiers from vegetable oils and industrial wastes. Results Trichosporon mycotoxinivorans CLA2 is bioemulsifier-producing yeast strain isolated from effluents of the dairy industry, with ability to emulsify different hydrophobic substrates. Bioemulsifier production (mg/L) and the emulsifying activity (E24) of this strain were optimized by response surface methodology using mineral minimal medium containing refinery waste as the carbon source, which consisted of diatomaceous earth impregnated with esters from filters used in biodiesel purification. The highest bioemulsifier production occurred in mineral minimal medium containing 75 g/L biodiesel residue and 5 g/L ammonium sulfate. The highest emulsifying activity was obtained in medium containing 58 g/L biodiesel refinery residue and 4.6 g/L ammonium sulfate, and under these conditions, the model estimated an emulsifying activity of 85%. Gas chromatography and mass spectrometry analysis suggested a bioemulsifier molecule consisting of monosaccharides, predominantly xylose and mannose, and a long chain aliphatic groups composed of octadecanoic acid and hexadecanoic acid at concentrations of 48.01% and 43.16%, respectively. The carbohydrate composition as determined by GC-MS of their alditol acetate derivatives showed a larger ratio of xylose (49.27%), mannose (39.91%), and glucose (10.81%). 1 H NMR spectra confirmed by COSY suggested high molecular weight, polymeric pattern, presence of monosaccharide’s and long chain aliphatic groups in the bioemulsifier molecule. Conclusions The biodiesel residue is an economical substrate, therefore seems to be very promising for the low-cost production of active emulsifiers in the emulsification of aromatics, aliphatic hydrocarbons, and kerosene.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBioMed Central Ltd.
dc.rightsopen access
dc.subjectBioemulsifier
dc.subjectYeasts
dc.subjectResponse surface methodology
dc.subjectBiodiesel residue
dc.subjectDiatomaceous earth
dc.titleBioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2
dc.typeArticle


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