Performance of microvesicles as biomarkers of clinical outcome in sepsis and trauma: A pilot study

dc.creatorPuga, Marcelo Lourencini
dc.creatorMenegueti, Mayra Gonçalves
dc.creatorSilvestrini, Marina Malheiros Araújo
dc.creatorSantos, Lorena Júnia de Souza
dc.creatorNogueira, Raquel Ferreira
dc.creatorBasile Filho, Anibal
dc.creatorCarvalho, Andréa Teixeira de
dc.creatorMartins Filho, Olindo Assis
dc.creatorMartins, Maria Auxiliadora
dc.date2023-01-23T18:24:55Z
dc.date2023-01-23T18:24:55Z
dc.date2022
dc.date.accessioned2023-09-26T23:13:54Z
dc.date.available2023-09-26T23:13:54Z
dc.identifierPUGA, Marcelo Lourencini et al. Performance of microvesicles as biomarkers of clinical outcome in sepsis and trauma: A pilot study. Biomed Pharmacother., v. 146, 112490, 2022. doi: 10.1016/j.biopha.2021.112490.
dc.identifier0753-3322
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/56562
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8888291
dc.descriptionSepsis remains one of the main causes of death in intensive care unit (ICU) worldwide, despite all technological and scientific advances. Microvesicles (MV) have become promising biomarkers for quick and accurate monitoring of several illnesses. The aim of this pilot study was to characterize and evaluate the performance of MV as biomarker of clinical outcome in septic and trauma patients. For this purpose, 39 subjects, both genders, aging from 18 to 85 years were included in three groups referred as Sepsis, Trauma and Healthy Control. Kinetic analysis of MV was carried out at four consecutive time points: admission (baseline)/T1, 24 h/T2, 72 h/T3 and outcome/T4 of discharge or death. At admission, an overall increase in total MV (Annexin V+) was observed in Sepsis.MV CD14+ (monocytes) was a putative biomarker to identify trauma patients, while MV CD3+ (T-cells) and CD41+ (platelets) were qualified to discriminated Trauma from Sepsis. Sepsis (Death) presented an increase in MV Annexin V+, CD45+, CD16+, CD14+, and CD41+ in comparison to Sepsis (Discharge). Moreover, Trauma (Death) presented an increase of MV CD3+ and CD235+ as compared to Trauma (Discharge). Analysing the ROC curve of specific MV evaluated according to performance, an accuracy of 100% was found to segregate the outcome in sepsis, and 95% in trauma. Our findings suggest that MV might be useful as a potential role in discriminating outcome in patients with sepsis/septic shock and trauma with high accuracy. However, further studies with a larger number of participants will be necessary to validate our findings.
dc.formatapplication/pdf
dc.languageeng
dc.publisherEditions Scientifiques Elsevier
dc.rightsopen access
dc.subjectMicrovesicles
dc.subjectSepsis
dc.subjectSeptic shock
dc.subjectBiomarkers
dc.subjectTrauma
dc.titlePerformance of microvesicles as biomarkers of clinical outcome in sepsis and trauma: A pilot study
dc.typeArticle


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