Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration

dc.creatorSilva, Marcelo S. da
dc.creatorCayres-Silva, Gustavo R.
dc.creatorVitarelli, Marcela O.
dc.creatorMarin, Paula A.
dc.creatorHiraiwa, Priscila Mazzocchi
dc.creatorAraújo, Christiane B.
dc.creatorScholl, Bruno B.
dc.creatorÁvila, Andréa Rodrigues
dc.creatorMcCulloch, Richard
dc.creatorReis, Marcelo S.
dc.creatorElias, Maria Carolina
dc.date2021-01-19T17:22:41Z
dc.date2021-01-19T17:22:41Z
dc.date2020
dc.date.accessioned2023-09-26T23:13:41Z
dc.date.available2023-09-26T23:13:41Z
dc.identifierSILVA, Marcelo S. da et al. Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration. PLoS ONE, v.9, n. 18512, p. 1–19, 2019.
dc.identifier2045-2322
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/45739
dc.identifierdoi.org/10.1038/s41598-019-54366-w
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8888249
dc.descriptionThe co-synthesis of DNA and RNA potentially generates conficts between replication and transcription, which can lead to genomic instability. In trypanosomatids, eukaryotic parasites that perform polycistronic transcription, this phenomenon and its consequences are still little studied. Here, we showed that the number of constitutive origins mapped in the Trypanosoma brucei genome is less than the minimum required to complete replication within S-phase duration. By the development of a mechanistic model of DNA replication considering replication-transcription conficts and using immunofuorescence assays and DNA combing approaches, we demonstrated that the activation of non-constitutive (backup) origins are indispensable for replication to be completed within S-phase period. Together, our fndings suggest that transcription activity during S phase generates R-loops, which contributes to the emergence of DNA lesions, leading to the fring of backup origins that help maintain robustness in S-phase duration. The usage of this increased pool of origins, contributing to the maintenance of DNA replication, seems to be of paramount importance for the survival of this parasite that afects million people around the world.
dc.formatapplication/pdf
dc.languagepor
dc.publisherNature Research
dc.rightsopen access
dc.subjectDNA Replication
dc.subjectTranscription, Genetic
dc.subjectS Phase
dc.subjectReplicación del ADN
dc.subjectTranscripción Genética
dc.subjectRéplication de l'ADN
dc.subjectTranscription génétique
dc.subjectPhase S
dc.subjectReplicação do DNA
dc.subjectTrypanosoma
dc.subjectTranscrição Genética
dc.subjectTrypanosoma brucei
dc.subjectFase S
dc.titleTranscription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration
dc.typeArticle


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