Comparison of viral elution-concentration methods for recovering noroviruses from deli meats

dc.creatorLuz, Isabelle da Silva
dc.creatorMiagostovich, Marize Pereira
dc.date2019-05-09T12:26:52Z
dc.date2019-05-09T12:26:52Z
dc.date2018
dc.date.accessioned2023-09-26T23:13:38Z
dc.date.available2023-09-26T23:13:38Z
dc.identifierLUZ, Isabelle da Silva; MIAGOSTOVICH, Marise Pereira. Comparison of viral elution-concentration methods for recovering noroviruses from deli meats. Journal of Virological Methods, v. 260, p. 49–55, 2018.
dc.identifier0166-0934
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/33006
dc.identifier10.1016/j.jviromet.2018.07.001
dc.identifier1879-0984
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8888241
dc.descriptionThis study aimed to assess viral elution-concentration methods for recovering noroviruses from deli meats. Spiking experiments were conducted to evaluate the recovery success rates and recovery efficiencies of human norovirus (NoV) GI and GII and murine norovirus 1 (MNV-1) using polyethylene glycol (PEG 6000) precipitation, skimmed milk flocculation (SMF), TRIzol® reagent, and a combination of PEG/TRIzol® and SMF/TRIzol® methods. Molecular analysis using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) revealed TRIzol® as the best method to be used for viral recovery from ham with medium recovery rates of 37.6% for NoV GI and 50.1% for NoV GII. Viral recovery from turkey meat showed medium recovery rates of 14.4% for NoV GI and 8.9% for NoV GII. For MNV-1, the rates varied from 0.5% to 80.8% not only according to the matrix but also with the associated virus and its inoculum (NoV GI or GII). The monitoring of commercial samples obtained in the Great Metropolitan region of Rio de Janeiro in order to demonstrate the occurrence of NoV GI and GII contamination in both matrices was also performed in 60 samples. NoV GI or GII were not detected in any samples, while MNV-1 used as the sample process control viruswas successfully recovered in 100% of samples.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectNorovirus
dc.subjectMétodos de concentração de eluição viral
dc.subjectPresunto
dc.subjectProduzir segurança
dc.subjectCarne de peru
dc.subjectReação em Cadeia da Polimerase em Tempo Real
dc.subjectNorovirus
dc.subjectViral elution-concentration methods
dc.subjectRT-qPCR
dc.subjectHam
dc.subjectTurkey meat
dc.subjectProduce safety
dc.subjectReação em Cadeia da Polimerase em Tempo Real
dc.titleComparison of viral elution-concentration methods for recovering noroviruses from deli meats
dc.typeArticle


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