BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure

dc.creatorSilva Filho, Benisio Ferreira
dc.creatorFilipak Neto, Francisco
dc.creatorMarchi, Micheli de
dc.creatorMoggio, Erick Laurent
dc.creatorRossi, Izadora Volpato
dc.creatorSabatke, Bruna
dc.creatorRamírez, Marcel Ivan
dc.creatorLucena, Miguel Clodomiro dos Santos
dc.creatorTodeschini, Adriane Regina
dc.creatorRibeiro, Ciro Alberto de Oliveira
dc.date2022-11-25T15:19:42Z
dc.date2022-11-25T15:19:42Z
dc.date2022
dc.date.accessioned2023-09-26T23:13:29Z
dc.date.available2023-09-26T23:13:29Z
dc.identifierSILVA FILHO, Benisio Ferreira et al. BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure. Environmental Pollution, v. 313, n. 120140, p. 1–10 2022.
dc.identifier1873-6424
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/55793
dc.identifierhttps://doi.org/10.1016/j.envpol.2022.120140
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8888212
dc.descriptionTCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and BDE-209 (decabromodiphenyl ether) are persistent organic pollutants (POPs) produced by industrial activities and associated with several diseases. TCDD is a known human carcinogen, but few studies investigated about the effects of exposure to both compounds, i.e., whether BDE-209 and TCDD can render tumor cells more aggressive and metastatic. In the current study we investigated if the exposure of B16–F1 and B16–F10 melanoma murine cells to environmental relevant concentrations of TCDD and BDE-209 at 24 h and 15-day exposure modulates the expression of genes related to metastasis, making the cells more aggressive. Both pollutants did not affect cell viability but lead to increase of cell proliferation, including the upregulation of vimentin, MMP2, MMP9, MMP14 and PGK1 gene expression and downregulation of Ecadherin, TIMP2, TIMP3 and RECK, strongly suggesting changes in cell phenotypes defined as epithelial to mesenchymal transition (EMT) in BDE-209 and TCDD-exposed cells. Foremost, increased expression of metalloproteinases and decreased expression of their inhibitors made B16–F1 cells similar the more aggressive B16–F10 cell line. Also, the higher secretion of extracellular vesicles by cells after acute exposure to BDE-209 could be related with the phenotype changes. These results are a strong indication of the potential of BDE209 and TCDD to modulate cell phenotype, leading to a more aggressive profile.
dc.formatapplication/pdf
dc.languagepor
dc.publisherElsevier
dc.rightsopen access
dc.subjectTCDD
dc.subjectBDE-219
dc.subjectMetalloproteases
dc.subjectEpithelial-Mesenchymal Transition
dc.subjectExtracellular Vesicles
dc.subject2,3,7,8-Tetraclorodibenzo-p-dioxina
dc.subjectHydrocarbons, Brominated
dc.subjectDioxins
dc.subjectCarcinogenesis
dc.subjectMetaloproteasas
dc.subjectTransición Epitelial-Mesenquimal
dc.subjectVesículas Extracelulares
dc.subjectDibenzodioxinas Policloradas
dc.subjectHidrocarburos Bromados
dc.subjectCarcinogénesis
dc.subjectMetaloproteases
dc.subjectTransição Epitelial-Mesenquimal
dc.subjectVesículas Extracelulares
dc.subjectDibenzodioxinas Policloradas
dc.subjectHidrocarbonetos Bromados
dc.subjectDioxinas
dc.subjectCarcinogênese
dc.titleBDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure
dc.typeArticle


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