Activation of transforming growth factor beta by Trypanosoma cruzi

dc.creatorWaghabi, M. C.
dc.creatorKeramidas, M.
dc.creatorFeige, J. J.
dc.creatorAraujo-Jorge, Tania C.
dc.creatorBailly, S.
dc.date2019-12-12T15:54:03Z
dc.date2019-12-12T15:54:03Z
dc.date2005
dc.date.accessioned2023-09-26T23:12:12Z
dc.date.available2023-09-26T23:12:12Z
dc.identifierWAGHABI, M. C. et al. Activation of transforming growth factor b by Trypanosoma cruzi. Celular Microbiology, v. 7, n. 4, p. 511-517, 2005.
dc.identifier1462-5814
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/37911
dc.identifier10.1111/j.1462-5822.2004.00481.x
dc.identifier1462-582
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887973
dc.descriptionThe anti-inflammatory cytokine, transforming growth factor beta (TGFbeta), plays an important role in Chagas disease, which is caused by the protozoan parasite Trypanosoma cruzi. In the current study, we show that the addition of an anti-TGFbeta antibody inhibited T. cruzi infection of cardiomyocytes, demonstrating the requirement for active endogenous TGFbeta. As TGFbeta is synthesized as a biologically inactive precursor, which is proteolytically processed to yield a mature, active homodimer, we hypothesized that T. cruzi could activate latent TGFbeta. To test this, we added recombinant latent TGFbeta to a TGFbeta-responsive reporter cell line in the presence of T. cruzi. We observed that T. cruzi was able to activate latent recombinant TGFbeta in this cellular model. We then investigated the ability of T. cruzi to activate latent TGFbetain vitro. We found that live T. cruzi, or cytosolic extracts of T. cruzi, activated latent TGFbeta in a dose- and temperature-dependent manner. The agent involved in TGFbeta activation was shown to be thermolabile and hydrophobic. Taken together, our studies demonstrate that T. cruzi directly activates latent TGFbeta. This activation is required for parasite entry into the mammalian cells and is likely to play an important role in modulating the outcome of T. cruzi infection.
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley 12 Months
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectTransformando o fator de crescimento b
dc.subjectAtivação
dc.subjectTGFbeta
dc.subjectTrypanosoma cruzi
dc.subjectTransforming growth factor b
dc.subjectActivation
dc.subjectTGFbeta
dc.titleActivation of transforming growth factor beta by Trypanosoma cruzi
dc.typeArticle


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