Multiplex PCR-based detection of leptospira in environmental water samples obtained from a slum settlement

dc.creatorVital-Brazil, Juliana Magalhães
dc.creatorBalassiano, Ilana Teruszkin
dc.creatorOliveira, Fabiano Sutter de
dc.creatorCosta, Alberto Dias de Souza
dc.creatorHillen, Leandro
dc.creatorPereira, Martha Maria
dc.date2018-03-13T16:17:15Z
dc.date2018-03-13T16:17:15Z
dc.date2010
dc.date.accessioned2023-09-26T23:11:29Z
dc.date.available2023-09-26T23:11:29Z
dc.identifierVITAL-BRAZIL, Juliana Magalhães; et al. Multiplex PCR-based detection of leptospira in environmental water samples obtained from a slum settlement. Mem Inst Oswaldo Cruz, v.105, n.3, p.353-355, May 2010.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/25284
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887841
dc.descriptionThe aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectLeptospira
dc.subjectReação em Cadeia da Polimerase Multiplex
dc.subjectamostra de água
dc.subjectLeptospira spp
dc.subjectmultiplex PCR
dc.subjectwater sample
dc.titleMultiplex PCR-based detection of leptospira in environmental water samples obtained from a slum settlement
dc.typeArticle


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