Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals

dc.creatorNeves, Neuza Maria Alcântara
dc.creatorSantos, Ricardo Ribeiro dos
dc.creatorAmor, Ana Lúcia Moreno
dc.creatorUemurac, Haruki
dc.creatorSilva Neto, Samuel J.
dc.creatorEichinger, Daniel
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.date2014-09-08T12:29:20Z
dc.date2014-09-08T12:29:20Z
dc.date2004
dc.date.accessioned2023-09-26T23:11:22Z
dc.date.available2023-09-26T23:11:22Z
dc.identifierNEVES, Neuza Maria Alcântara et al. Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals. Microbial Pathogenesis, v. 37, n. 5, p. 273-278, 2004.
dc.identifier0882-4010
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8311
dc.identifier10.1016/j.micpath.2004.03.003
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887817
dc.descriptionTrypanosoma cruzi is an obligate intracellular protozoan parasite that actively penetrates into non-phagocytic mammalian cells. To accomplish this, the parasite relies on the binding of cell surface ligands. It is reported herein that the T. cruzi trans-sialidase (TS), which is exposed on the parasite surface, binds to mouse heart cells, and should therefore be further studied as a possible cell penetration-related ligand. In addition, as has been proposed elsewhere, the binding of T. cruzi to tissues may turn them into targets for parasite-specific immune reactions. Washed heart sections from T. cruzi-infected mice were subjected to immunoenzymatic staining with antisera against whole T. cruzi and with polyclonal or monoclonal antibodies against TS. The anti-TS antibodies stained both parasites and uninfected heart cells in the vicinity of T. cruzi nest remains/trypomastigotes. On the other hand, an anti-T. cruzi serum, which did not recognize TS, only stained the parasites. In addition, normal heart sections from uninfected nude mice were shown to react with both enzymatically active and inactive recombinant TS molecules, probably through their amino-terminal region, since a recombinant TS lacking this region failed to bind.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectTrans-sialidase
dc.subjectBinding
dc.subjectHeart tissue
dc.subjectMiocárdio/metabolismo
dc.subjectMiócitos Cardíacos/metabolismo
dc.subjectNeuraminidase/metabolismo
dc.subjectTrypanosoma cruzi/enzimologia
dc.subjectAnimais
dc.subjectAnticorpos Antiprotozoários/sangue
dc.subjectGlicoproteínas
dc.subjectCamundongos Endogâmicos BALB C
dc.subjectCamundongos
dc.subjectCamundongos Nus
dc.subjectMiócitos Cardíacos/citologia
dc.subjectMiócitos Cardíacos/parasitologia
dc.subjectTrypanosoma cruzi/imunologia
dc.subjectVirulência
dc.titleParasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals
dc.typeArticle


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