Schizodeme analysis of Trypanosoma cruzi stocks from South and Central America by analysis of PCR-amplified minicircle variable region sequences

dc.creatorAvila, Herbert
dc.creatorGonçalves, Antonio M.
dc.creatorNehme, Nedia Saad
dc.creatorMorel, Carlos Medicis
dc.creatorSimpson, Larry
dc.date2016-06-29T14:13:31Z
dc.date2016-07-07T11:44:14Z
dc.date2016-06-29T14:13:31Z
dc.date2016-07-07T11:44:14Z
dc.date1990
dc.date.accessioned2023-09-26T23:10:43Z
dc.date.available2023-09-26T23:10:43Z
dc.identifierAVILA, Herbert et al. Schizodeme analysis of Trypanosoma cruzi stocks from South and Central America by analysis of PCR-amplified minicircle variable region sequences. Molecular and Biochemical Parasitology, v. 42, p. 175-188, 1990.
dc.identifier0166-6851
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/14612
dc.identifier10.1016/0166-6851(90)90160-N
dc.identifier1872-9428
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887709
dc.descriptionKinetoplast DNA (kDNA) was isolated from 56 stocks of Trypanosoma cruzi isolated from human patients, animals and insects from Brazil, Venezuela, Colombia and Costa Rica. Comparison of the patterns of digested kDNA on acrylamide gels led to the grouping of several stocks into two schizodemes. Schizodeme analysis was also performed using a set of 330-bp fragments representing all the variable regions of the minicircle DNA molecules, which were obtained by PCR amplification of the kDNA using conserved region primers. The results of this analysis were consistent with the analysis using total kDNA, but the more informative restriction profiles allowed the construction of additional schizodemes. In addition, two oligomers were generated from variable region sequences of cloned minicircles from a Y and a CI strain, and these were used as schizodeme-specific probes to detect homologous sequences in the amplified minicircle DNAs. The results indicate that a combination of restriction enzyme fingerprinting and hybridization of amplified variable region minicircle DNA with schizodeme-specific probes can be used for both sensitive detection and classification of T. cruzi.
dc.formatapplication/pdf
dc.languagepor
dc.publisherElsevier
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectPolymerase chain reaction
dc.subjectSchizodeme
dc.subjectMinicircle DNA
dc.subjectTrypanosoma cruzi
dc.subjectReação em Cadeia da Polimerase
dc.titleSchizodeme analysis of Trypanosoma cruzi stocks from South and Central America by analysis of PCR-amplified minicircle variable region sequences
dc.typeArticle


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