Assessment of the IgA immunoassay diagnostic potential of the Mycobacterium tuberculosis MT10.3-MPT64 fusion protein in tuberculous pleural fluid

dc.creatorAraujo, Leonardo Silva
dc.creatorMoraes, Renata Maciel
dc.creatorTrajman, Anete
dc.creatorSaad, Maria Helena Féres
dc.date2018-04-26T15:03:18Z
dc.date2018-04-26T15:03:18Z
dc.date2010
dc.date.accessioned2023-09-26T23:10:37Z
dc.date.available2023-09-26T23:10:37Z
dc.identifierARAUJO, Leonardo Silva; et al. Assessment of the IgA Immunoassay Diagnostic Potential of the Mycobacterium tuberculosis MT10.3-MPT64 Fusion Protein in Tuberculous Pleural Fluid. Clinical and Vaccine Immunology, v.17, n.2, p.1963-1969, Dec. 2010.
dc.identifier1556-6811
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26136
dc.identifier10.1128/CVI.00372-10
dc.identifier1556-679X
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887690
dc.descriptionPleural tuberculosis (PL-TB) remains difficult to diagnose. An enzyme-linked immunosorbent assay (ELISA) was developed based on a construction containing the fusion of the Rv3019c (MT10.3) and Rv1980c (MPT64) gene sequences, and its performance was evaluated in an area where TB is endemic. A total of 92 pleural fluid (PF) samples at serial dilutions of 1:50 to 1:800 were included in the ELISA IgA MT10.3-MPT64 evaluation: 70 from TB patients and 22 from patients with other pleurisies. Confirmation of the expression and subsequent purification of the protein was made by SDS-PAGE and Western blot assays, resulting in a 36-kDa protein. ELISA IgA MT10.3-MPT64 showed sensitivities of 61.4%, 58.6%, 62.9%, 67.1%, and 70% at each PF dilution, respectively. The cumulative results of all dilutions increased sensitivity to 81.4% without jeopardizing specificity. Similar results were also obtained at the combined dilutions of 1:50, 1:200, and 1:800 or 1:50 plus 1:800 dilutions (80%). The overall sensitivity of the reference test, i.e., histopathological examination, was 74%. But, via the ELISA IgA MT10.3-MPT64 test, sensitivity was high for specimens with a negative culture (23/27; 85.2%) or nonspecific histopathology (17/18; 94.4%). Our findings demonstrated the promising use of this test as an adjunct in PL-TB diagnoses, particularly in cases with lower bacterial loads and false-negative results in the reference tests, since the new test includes such important features as quick and easy application, high sensitivity and, perhaps most importantly, affordability, which is so crucial for its widespread use in developing countries.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Microbiology
dc.rightsopen access
dc.subjectMycobacterium tuberculosis
dc.subjectFluido Pleural Tuberculoso
dc.subjectImunoensaio IgA
dc.subjectDiagnóstico
dc.subjectMycobacterium tuberculosis
dc.subjectTuberculous Pleural Fluid
dc.subjectIgA Immunoassay Diagnostic
dc.subjectDiagnostic
dc.titleAssessment of the IgA immunoassay diagnostic potential of the Mycobacterium tuberculosis MT10.3-MPT64 fusion protein in tuberculous pleural fluid
dc.typeArticle


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