Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis

dc.creatorBarbosa, Artur F
dc.creatorSangiorgi, Bruno B
dc.creatorGaldino, Suely L
dc.creatorBarral Netto, Manoel
dc.creatorPitta, Ivan R
dc.creatorPinheiro, Antonio Luiz Barbosa
dc.date2014-02-25T13:29:22Z
dc.date2014-02-25T13:29:22Z
dc.date2012
dc.date.accessioned2023-09-26T23:09:18Z
dc.date.available2023-09-26T23:09:18Z
dc.identifierBARBOSA, A. F. et al. Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis. Lasers in Surgery and Medicine, v. 44, n. 10, p. 850-855, 2012.
dc.identifier1096-9101
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7350
dc.identifier10.1002/lsm.22099
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887455
dc.descriptionCutaneous and mucocutaneous leishmaniasis are diseases characterized by skin or mucosal manifestations. In the new world, Leishmania braziliensis is the main etiological agent of cutaneous leishmaniasis, condition that may evolve to the mucocutaneous form. The therapeutic arsenal routinely employed to treat infected patients is unsatisfactory, especially for pentavalent antimonials, treatment recommended by the WHO, as they are often highly toxic, poorly tolerated and of variable effectiveness. This work aimed to evaluate in vitro the effectiveness of photodynamic antimicrobial chemotherapy as a new approach for the treatment of leishmaniasis. MATERIALS AND METHODS: A laser (λ = 660 nm, 40 mW, 4.2 J/cm2 , and 8.4 J/cm2 , CW) associated to phenothiazine's derivatives (5 and 10 µg/ml, toluidine blue O, methylene blue, or phenothiazine) on the promastigote forms of L. braziliensis in a single session. Samples were removed and analyzed in a hemocytometer 72 hours after PACT and viability of the parasites was assessed in quadruplicates. RESULTS: An important decrease in the number of viable parasites on all treated groups in comparison to their controls was observed as all tested compounds lead to significant parasite lethality being the highest lethality achieved with 10 µg/ml of TBO. No lethality was observed on groups treated with laser or with any of the compounds separately. CONCLUSIONS: TBO presented higher parasite lethality in comparison to MB with impressive reduction from 1 hour to 5 minutes of pre-incubation time.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley Periodicals, Inc
dc.rightsopen access
dc.subjectLaser-therapy
dc.subjectLeishmaniasis
dc.subjectMethylene blue
dc.subjectToluidine blue O
dc.subjectTreatment
dc.subjectLeishmania braziliensis/efeitos de drogas
dc.subjectLeishmaniose Cutânea/quimioterapia
dc.subjectAzul de Metileno/uso terapêutico
dc.subjectFotoquimioterapia
dc.subjectFármacos Fotossensibilizantes/uso terapêutico
dc.subjectCloreto de Tolônio/uso terapêutico
dc.subjectTripanossomicidas/uso terapêutico
dc.subjectHumanos
dc.subjectAzul de Metileno/farmacologia
dc.subjectFenotiazinas/farmacologia
dc.subjectFenotiazinas/uso terapêutico
dc.subjectFármacos Fotossensibilizantes/farmacologia
dc.subjectCloreto de Tolônio/farmacologia
dc.subjectTripanossomicidas/farmacologia
dc.titlePhotodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis
dc.typeArticle


Este ítem pertenece a la siguiente institución