Evaluation of monocyte subsets and markers of activation in leprosy reactions.

dc.creatorShibuya, Mayume
dc.creatorBergheme, Giovana
dc.creatorPassos, Sara Timoteo
dc.creatorQueiroz, Ivonete
dc.creatorRêgo, Jamile
dc.creatorCarvalho, Lucas Pedreira de
dc.creatorMachado, Paulo Roberto Lima
dc.date2018-12-18T16:51:46Z
dc.date2018-12-18T16:51:46Z
dc.date2018
dc.date.accessioned2023-09-26T23:09:09Z
dc.date.available2023-09-26T23:09:09Z
dc.identifierSHIBUYA, M. et al. Evaluation of monocyte subsets and markers of activation in leprosy reactions. Microbes and Infection, oct. 2018.
dc.identifier1286-4579
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30673
dc.identifier10.1016/j.micinf.2018.10.003
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887426
dc.descriptionInstituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), Brazil.
dc.descriptionUnderstanding host immune pathways associated with tissue damage during reactions are of upmost importance to the development of immune intervention strategies. The participation of monocytes in leprosy reactions was evaluated by determining the frequency of monocyte subsets and the degree of cellular activation through the expression of MHCII and the co-stimulatory molecules CD40, CD80, CD86. Leprosy subjects with or without reactions were included in this cross-sectional study. Peripheral blood mononuclear cell were isolated and stained ex vivo to determine monocyte subsets and the degree of cellular activation by flow cytometry. Intermediate monocytes were increased in leprosy patients with reactions when compared to patients without reactions. Although no difference was detected in the frequency of monocyte subsets between type 1 and 2 reactions, the expression of CD80 was increased in monocytes from patients with type 1 reactions and CD40 was higher in paucibacillary subjects presenting type 1 reactions. Moreover, CD86 and MHC II expression were higher in intermediate monocytes when compared to the other subsets in leprosy reaction types 1 and 2. Intermediate monocyte activation with CD86 and MHCII expression is involved with both type 1 and 2 reactions, whereas CD80 and CD40 expression is related to type 1 reactions.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsopen access
dc.subjectHanseníase
dc.subjectReações de lepra
dc.subjectMonócitos
dc.subjectMoléculas co-estimulatórias
dc.subjectLeprosy
dc.subjectLeprosy reactions
dc.subjectMonocytes
dc.subjectCo-stimulatory molecules
dc.titleEvaluation of monocyte subsets and markers of activation in leprosy reactions.
dc.typeArticle


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