Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteins

dc.creatorBonilauri, Bernardo
dc.creatorHoletz, Fabíola Barbieri
dc.creatorDallagiovanna, Bruno
dc.date2021-12-13T19:08:03Z
dc.date2021-12-13T19:08:03Z
dc.date2021
dc.date.accessioned2023-09-26T23:08:58Z
dc.date.available2023-09-26T23:08:58Z
dc.identifierBONILAURI, Bernardo et al. Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: From RNAs to microproteins. Biomolecules, v.11, n. 1673, p. 1–17, 2021.
dc.identifier2218-273X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/50365
dc.identifier10.3390/biom11111673
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887390
dc.descriptionRibosome profiling reveals the translational dynamics of mRNAs by capturing a ribosomal footprint snapshot. Growing evidence shows that several long non-coding RNAs (lncRNAs) contain small open reading frames (smORFs) that are translated into functional peptides. The difficulty in identifying bona-fide translated smORFs is a constant challenge in experimental and bioinformatics fields due to their unconventional characteristics. This motivated us to isolate human adipose-derived stem cells (hASC) from adipose tissue and perform a ribosome profiling followed by bioinformatics analysis of transcriptome, translatome, and ribosome-protected fragments of lncRNAs. Here, we demonstrated that 222 lncRNAs were associated with the translational machinery in hASC, including the already demonstrated lncRNAs coding microproteins. The ribosomal occupancy of some transcripts was consistent with the translation of smORFs. In conclusion, we were able to identify a subset of 15 lncRNAs containing 35 smORFs that likely encode functional microproteins, including four previously demonstrated smORF-derived microproteins, suggesting a possible dual role of these lncRNAs in hASC self-renewal.
dc.formatapplication/pdf
dc.languagepor
dc.publisherMDPI
dc.rightsopen access
dc.subjectMicroproteína
dc.subjectlncRNA
dc.subjectRNA, Long Noncoding
dc.subjectRibosomes
dc.subjectOpen Reading Frames
dc.subjectMicroprotein
dc.subjectProtein Biosynthesis
dc.subjectStem Cells
dc.subjectARN Largo no Codificante
dc.subjectRibosomas
dc.subjectSistemas de Lectura Abierta
dc.subjectBiosíntesis de Proteínas
dc.subjectCélulas Madre
dc.subjectARN long non codant
dc.subjectRibosomes
dc.subjectCadres ouverts de lecture
dc.subjectBiosynthèse des protéines
dc.subjectCellules souches
dc.subjectRNA Longo não Codificante
dc.subjectRibossomos
dc.subjectFases de Leitura Aberta
dc.subjectBiossíntese de Proteínas
dc.subjectCélulas-Tronco
dc.titleLong non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteins
dc.typeArticle


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