Short interspersed repetitive elements (SINEs) from the cichlid fish, Oreochromis niloticus, and their chromosomal localization by fluorescent in situ hybridization

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOliveira, Claudio
dc.creatorWang, Yimin
dc.creatorBryden, Louis J.
dc.creatorWright, Jonathan M.
dc.date2014-05-27T11:20:38Z
dc.date2016-10-25T18:18:32Z
dc.date2014-05-27T11:20:38Z
dc.date2016-10-25T18:18:32Z
dc.date2003-04-01
dc.date.accessioned2017-04-06T01:05:23Z
dc.date.available2017-04-06T01:05:23Z
dc.identifierCaryologia, v. 56, n. 2, p. 181-189, 2003.
dc.identifier0008-7114
dc.identifierhttp://hdl.handle.net/11449/67242
dc.identifierhttp://acervodigital.unesp.br/handle/11449/67242
dc.identifier10.1080/00087114.2003.10589323
dc.identifierWOS:000184285700008
dc.identifier2-s2.0-0141679003
dc.identifierhttp://dx.doi.org/10.1080/00087114.2003.10589323
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/888720
dc.descriptionIn the present study, we describe the cloning and characterization of a new SINE-like element from O. niloticus (ROn-2) and show the distribution of this SINE and a previously isolated SINE, ROn-1, in the chromosomes of O. niloticus. The ROn-2 element is 359 base pairs (bp) in length, contains short direct terminal repeats, a tRNA-related region similar to tRNA Val and tRNA Arg, a tRNA-unrelated region, and a poly-A tail. Analysis of the chromosomal distribution of ROn-1 and ROn-2 by fluorescent in situ hybridization showed that both SINE sequences are present in all chromosomes of tilapia, and organized in small clusters. The only exception was a large cluster of ROn-1 repeats found in the middle of the long arm of chromosome 1. In view of our data we discuss the hypothesis that the absence of large clusters of SINE sequences and the structural composition of these sequences may explain the absence of base-specific fluorochrome bands in the chromosomes of tilapia.
dc.languageeng
dc.relationCaryologia
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectChromosome structure
dc.subjectFish
dc.subjectFluorescent in situ hybridization
dc.subjectRetrotransposon
dc.subjectSINE
dc.subjectCichlidae
dc.subjectOreochromis
dc.subjectOreochromis niloticus
dc.subjectTilapia
dc.subjecttransposons
dc.titleShort interspersed repetitive elements (SINEs) from the cichlid fish, Oreochromis niloticus, and their chromosomal localization by fluorescent in situ hybridization
dc.typeOtro


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