Characterization of a β-1,3-glucanase active in the alkaline midgut of Spodoptera frugiperda larvae and its relation to β-glucan-binding proteins

dc.creatorBragatto, Ivan
dc.creatorGenta, Fernando A.
dc.creatorRibeiro, Alberto F.
dc.creatorTerra, Walter R.
dc.creatorFerreira, Clélia
dc.date2018-12-20T11:09:17Z
dc.date2018-12-20T11:09:17Z
dc.date2010
dc.date.accessioned2023-09-26T23:07:55Z
dc.date.available2023-09-26T23:07:55Z
dc.identifierBRAGATTO, iVAN; et al. Characterization of a b-1,3-glucanase active in the alkaline midgut of Spodoptera frugiperda larvae and its relation to b-glucan-binding proteins. Insect Biochemistry and Molecular Biology, v.40, p.861-872, 2010.
dc.identifier0965-1748
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30709
dc.identifier10.1016/j.ibmb.2010.08.006
dc.identifier1879-0240
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887205
dc.descriptionSpodoptera frugiperda β-1,3-glucanase (SLam) was purified from larval midgut. It has a molecular mass of 37.5 kDa, an alkaline optimum pH of 9.0, is active against β-1,3-glucan (laminarin), but cannot hydrolyze yeast β-1,3-1,6-glucan or other polysaccharides. The enzyme is an endoglucanase with low processivity (0.4), and is not inhibited by high concentrations of substrate. In contrast to other digestive β-1,3-glucanases from insects, SLam is unable to lyse Saccharomyces cerevisae cells. The cDNA encoding SLam was cloned and sequenced, showing that the protein belongs to glycosyl hydrolase family 16 as other insect glucanases and glucan-binding proteins. Multiple sequence alignment of β-1,3-glucanases and β-glucan-binding protein supports the assumption that the β-1,3-glucanase gene duplicated in the ancestor of mollusks and arthropods. One copy originated the derived β-1,3-glucanases by the loss of an extended N-terminal region and the β-glucan-binding proteins by the loss of the catalytic residues. SLam homology modeling suggests that E228 may affect the ionization of the catalytic residues, thus displacing the enzyme pH optimum. SLam antiserum reacts with a single protein in the insect midgut. Immunocytolocalization shows that the enzyme is present in secretory vesicles and glycocalyx from columnar cells.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectProteínas de ligação ao glucano
dc.subjectB-1,3-glucanase de molusco
dc.subjectProcessividade
dc.subjectDeslocamento ótimo de pH
dc.subjectInsect b-1,3-glucanase
dc.subjectGlucan-binding proteins
dc.subjectpH optimum displacement
dc.subjectProcessivity
dc.subjectMolluskan b-1,3-glucanase
dc.titleCharacterization of a β-1,3-glucanase active in the alkaline midgut of Spodoptera frugiperda larvae and its relation to β-glucan-binding proteins
dc.typeArticle


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