Identification of Vibrio cholerae and Vibrio mimicus by multilocus sequence analysis (MLSA)

dc.creatorThompson, Cristiane C.
dc.creatorThompson, Fabiano L.
dc.creatorVicente, Ana Carolina P.
dc.date2019-04-16T16:33:51Z
dc.date2019-04-16T16:33:51Z
dc.date2008
dc.date.accessioned2023-09-26T23:06:34Z
dc.date.available2023-09-26T23:06:34Z
dc.identifierTHOMPSON, Cristiane C.; THOMPSON, Fabiano L.; VICENTE, Ana Carolina P. Identification of Vibrio cholerae and Vibrio mimicus by multilocus sequence analysis (MLSA). International Journal of Systematic and Evolutionary Microbiology, v. 58, p. 617–621, 2008.
dc.identifier1466-5026
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/32526
dc.identifier10.1099/ijs.0.65461-0
dc.identifier1466-5034
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886944
dc.descriptionVibrio cholerae and Vibrio mimicus have similar phenotypes and genomes making rapid differentiation of these two species difficult. The first standard multilocus sequence analysis (MLSA) scheme for the identification of these species is described. A collection of 45 representative isolates from different geographical regions and hosts was examined using segments of the housekeeping genes pyrH, recA and rpoA. Overall, the closest phylogenetic neighbours of these species were Vibrio furnissii and Vibrio fluvialis. V. cholerae and V. mimicus formed separate species clusters on the basis of each gene, suggesting that these genes are useful as identification markers. These species clusters arose by the accumulation of point mutations. The pyrH gene showed the highest resolution for differentiating V. cholerae and V. mimicus. The maximum interspecies pyrH gene sequence similarity was 91 %. Clearly, V. mimicus strains were more heterogeneous than V. cholerae strains at the three loci. It is suggested that vibrio species may be defined on the basis of MLSA data. A vibrio species was defined as a group of strains forming a monophyletic group on the basis of these loci and with an intraspecific sequence similarity of at least 95 %. V. cholerae and V. mimicus isolates can be readily identified through the open database resource ‘The Taxonomy of Vibrios’ (http://www.taxvibrio.lncc.br/).
dc.description2022-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherMicrobiology Society
dc.rightsrestricted access
dc.subjectVibrio cholerae
dc.subjectVibrio mimicus
dc.subjectTipagem de Sequências Multilocus
dc.subjectIdentificação
dc.subjectVibrio cholerae
dc.subjectVibrio mimicus
dc.subjectmultilocus sequence analysis
dc.subjectIdentification
dc.titleIdentification of Vibrio cholerae and Vibrio mimicus by multilocus sequence analysis (MLSA)
dc.typeArticle


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