Long-term administration of IgG2a anti-NK1.1 monoclonal antibody ameliorates lupus-like disease in NZB/W mice in spite of an early worsening induced by an IgG2a-dependent BAFF/BLyS production.

dc.creatorPostól, Edilberto
dc.creatorMeyer, André Villanova
dc.creatorCardillo, Fabíola
dc.creatorAlencar, Raquel de
dc.creatorPessina, Daniel Huber
dc.creatorNihei, Jorge Sadao
dc.creatorMariano, Mário
dc.creatorMengele Junior, José Orivaldo
dc.date2015-06-18T17:21:29Z
dc.date2015-06-18T17:21:29Z
dc.date2008
dc.date.accessioned2023-09-26T23:06:17Z
dc.date.available2023-09-26T23:06:17Z
dc.identifierPOSTÓL, E. et al. Long-term administration of IgG2a anti-NK1.1 monoclonal antibody ameliorates lupus-like disease in NZB/W mice in spite of an early worsening induced by an IgG2a-dependent BAFF/BLyS production. Immunology, v. 125, n. 2, p. 184-196, 2008.
dc.identifier1365-2567
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/10918
dc.identifier10.1111/j.1365-2567.2008.02835.x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886884
dc.descriptionThe role of natural killer (NK) T cells in the development of lupus-like disease in mice is still controversial. We treated NZB/W mice with anti- NK1.1 monoclonal antibodies (mAbs) and our results revealed that administration of either an irrelevant immunoglobulin G2a (IgG2a) mAb or an IgG2a anti-NK1.1 mAb increased the production of anti-dsDNA antibodies in young NZB/W mice. However, the continuous administration of an anti-NK1.1 mAb protected aged NZB/W mice from glomerular injury, leading to prolonged survival and stabilization of the proteinuria. Conversely, the administration of the control IgG2a mAb led to an aggravation of the lupus-like disease. Augmented titres of anti-dsDNA in NZB/ W mice, upon IgG2a administration, correlated with the production of BAFF/BLyS by dendritic, B and T cells. Treatment with an anti-NK1.1 mAb reduced the levels of interleukin-16, produced by T cells, in spleen cell culture supernatants from aged NZB/W. Adoptive transfer of NK T cells from aged to young NZB/W accelerated the production of antidsDNA in recipient NZB/W mice, suggesting that NK T cells from aged NZB/W are endowed with a B-cell helper activity. In vitro studies, using purified NK T cells from aged NZB/W, showed that these cells provided helper B-cell activity for the production of anti-dsDNA. We concluded that NK T cells are involved in the progression of lupus-like disease in mature NZB/W mice and that immunoglobulin of the IgG2a isotype has an enhancing effect on antibody synthesis due to the induction of BAFF/ BLyS, and therefore have a deleterious effect in the NZB/W mouse physiology.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.rightsopen access
dc.subjectBAFF/BLyS
dc.subjectFc receptor
dc.subjectInterleukin-16
dc.subjectNatural killer T cells
dc.subjectSystemic lupus erythematosus
dc.subjectToll-like recepto
dc.subjectAnticorpos Monoclonais/uso terapêutico
dc.subjectFator Ativador de Células B/biossíntese
dc.subjectImunoglobulina G/uso terapêutico
dc.subjectNefrite Lúpica/prevenção & controle
dc.subjectEnvelhecimento/imunologia
dc.subjectAnimais
dc.subjectAnticorpos Antinucleares/biossíntese
dc.subjectAnticorpos Monoclonais/imunologia
dc.subjectAntígenos Ly/imunologia
dc.subjectCélulas Cultivadas
dc.subjectProgressão da Doença
dc.subjectFeminino
dc.subjectImunoglobulina G/imunologia
dc.subjectInterleucina-16/biossíntese
dc.subjectCélulas Matadoras Naturais/imunologia
dc.subjectLipopolissacarídeos/imunologia
dc.subjectFígado/imunologia
dc.subjectNefrite Lúpica/imunologia
dc.subjectCamundongos
dc.subjectSubfamília B de Receptores Semelhantes a Lectina de Células
dc.subjectÍndice de Gravidade de Doença
dc.subjectLinfócitos T Auxiliares-Indutores/imunologia
dc.titleLong-term administration of IgG2a anti-NK1.1 monoclonal antibody ameliorates lupus-like disease in NZB/W mice in spite of an early worsening induced by an IgG2a-dependent BAFF/BLyS production.
dc.typeArticle


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