A killed Leishmania vaccine with sand fly saliva extract and saponin adjuvant displays immunogenicity in dogs

dc.creatorGiunchetti, Rodolfo Cordeiro
dc.creatorOliveira, Rodrigo Corrêa
dc.creatorMartins Filho, Olindo Assis
dc.creatorCarvalho, Andréa Teixeira de
dc.creatorRoatt, Bruno Mendes
dc.creatorSoares, Rodrigo Dian de Oliveira Aguiar
dc.creatorVital, Wendel Coura
dc.creatorAbreu, Raquel Tropia de
dc.creatorMalaquias, Luiz Cosme Cotta
dc.creatorGontijo, Nelder Figueiredo
dc.creatorBrodskyn, Claudia Ida
dc.creatorOliveira, Camila Indiani de
dc.creatorCosta, Dirceu Joaquim
dc.creatorLanag, Marta de
dc.creatorReis, Alexandre Barbosa
dc.date2014-09-17T18:49:23Z
dc.date2014-09-17T18:49:23Z
dc.date2008
dc.date.accessioned2023-09-26T23:06:12Z
dc.date.available2023-09-26T23:06:12Z
dc.identifierGIUNCHETTI, Rodolfo Cordeiro et al. A killed Leishmania vaccine with sand fly saliva extract and saponin adjuvant displays immunogenicity in dogs. Vaccine, v. 26, n. 5, p. 623-638, 2008.
dc.identifier0264-410X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/8391
dc.identifier10.1016/j.vaccine.2007.11.057
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886871
dc.descriptionA vaccine against canine visceral leishmaniasis (CVL), comprising Leishmania braziliensis promastigote protein, sand fly gland extract (SGE) and saponin adjuvant, was evaluated in dog model, in order to analyse the immunogenicity of the candidate vaccine. The vaccine candidate elicited strong antigenicity in dogs in respect of specific SGE and Leishmania humoral immune response. The major saliva proteins recognized by serum from immunized dogs exhibited molecular weights of 35 and 45 kDa, and were related to the resistance pattern against Leishmania infection. Immunophenotypic analysis revealed increased circulating CD21+ B-cells and CD5+ T-cells, reflected by higher counts of CD4+ and CD8+ T-cells. The observed interaction between potential antigen-presenting cells (evaluated as CD14+ monocytes) and lymphocyte activation status indicated a relationship between innate and adaptive immune responses. The higher frequency in L. chagasi antigen-specific CD8+ T-lymphocytes, and their positive association with intense cell proliferation, in addition to the progressively higher production of serum nitric oxide levels, showed a profile compatible with anti-CVL vaccine potential. Further studies on immunological response after challenge with L. chagasi may provide important information that will lead to a better understanding on vaccine trial and efficacy
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier Ltd
dc.rightsrestricted access
dc.subjectCanine visceral leishmaniasis
dc.subjectCell and humoral immune response
dc.subjectFlow cytometry
dc.subjectDoenças do Cão/imunologia
dc.subjectLeishmania braziliensis/imunologia
dc.subjectVacinas contra Leishmaniose/imunologia
dc.subjectLeishmaniose Visceral/imunologia
dc.subjectGlândulas Salivares/química
dc.subjectSaponinas/imunologia
dc.subjectExtratos de Tecidos/imunologia
dc.subjectAdjuvantes Imunológicos/administração & dosagem
dc.subjectAnimais
dc.subjectAnticorpos Antiprotozoários/sangue
dc.subjectAntígenos CD5
dc.subjectLinfócitos B/imunologia
dc.subjectReações Cruzadas
dc.subjectDoenças do Cão/sangue
dc.subjectFeminino
dc.subjectInjeções Subcutâneas
dc.subjectVacinas contra Leishmaniose/administração & dosagem
dc.subjectLeishmaniose Visceral/sangue
dc.subjectÓxido Nítrico/sangue
dc.subjectProteínas/química
dc.subjectSaponinas/administração & dosagem
dc.subjectLinfócitos T/imunologia
dc.titleA killed Leishmania vaccine with sand fly saliva extract and saponin adjuvant displays immunogenicity in dogs
dc.typeArticle


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