Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil

dc.creatorAndrade, M. K. N.
dc.creatorMachado, S. M. A.
dc.creatorLeite, M. L.
dc.creatorSaad, M. H. F.
dc.date2018-09-20T14:34:59Z
dc.date2018-09-20T14:34:59Z
dc.date2009
dc.date.accessioned2023-09-26T23:05:50Z
dc.date.available2023-09-26T23:05:50Z
dc.identifierANDRADE, M. K. N. et al. Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil. Brazilian Journal of medical and Biological Research, v.42, p.433-437, 2009.
dc.identifier0100-879X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28938
dc.identifier1414-431X
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886799
dc.descriptionAssuming that the IS6110-restriction fragment length polymorphism (RFLP) changes at a constant rate of 3.2 years, this methodology was applied to demonstrate, for the first time, variant patterns of Mycobacterium tuberculosis (MTB) in multiple isolates obtained at short time intervals from sputum and blood of an HIV+ patient with multiple admissions to the Emergency Room and to the multidrug-resistant tuberculosis (MDR-TB) Reference Center of a secondary-care hospital in Rio de Janeiro, Brazil. In sputum, the IS6110-RFLP appeared in isolates with two variant patterns with 10 and 13 IS6110 copies. However, blood presented only the pattern corresponding to 10 copies, suggesting compartmentalization. With regard to the exact match of 10 of 13 bands, this may be a subpopulation with the same clonal origin and this may be related to the IS6110 transposition. A susceptibility test demonstrated an MDR profile (INHR, RIFR, SMR, and EMBR), with the sputum isolate also exhibiting EMBS (R = resistant; S = sensitive). A gene mutation confirmed resistance only to streptomycin. There was agreement between the results of the phenotypic test and the clinical response to MDR-TB treatment, suggesting serious implications with regard to treatment administration based exclusively on molecular methods, and calling attention to the fact that more effective control strategies against the emergence of MDR strains must be implemented by the TB control program to prevent transmission of MDR-MTB strains at health facilities in areas highly endemic for TB.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.rightsopen access
dc.subjectTuberculose
dc.subjectMycobacterium tuberculosis
dc.subjectMutação de Gene
dc.subjectImpressão digital
dc.subjectTuberculosis
dc.subjectMycobacterium tuberculosis
dc.subjectTB-MDR
dc.subjectTB-RFLP
dc.subjectGene mutation
dc.subjectFingerprint
dc.titlePhenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
dc.typeArticle


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