Mycobacteria mobility shift assay: a method for the rapid identification of Mycobacterium tuberculosis and nontuberculous mycobacteria

dc.creatorWildner, Letícia Muraro
dc.creatorBazzo, Maria Luiza
dc.creatorLiedke, Susie Coutinho
dc.creatorNogueira, Christiane Lourenço
dc.creatorSegat, Gabriela
dc.creatorSenna, Simone Gonçalves
dc.creatorSchlindwein, Aline Daiane
dc.creatorOliveira, Jaquelline Germano de
dc.creatorRovaris, Darcita Bürger
dc.creatorBonjardim, Claudio Antonio
dc.creatorKroon, Erna Gessien
dc.creatorFerreira, Paulo César Peregrino
dc.date2015-02-27T12:56:22Z
dc.date2015-02-27T12:56:22Z
dc.date2014
dc.date.accessioned2023-09-26T23:05:48Z
dc.date.available2023-09-26T23:05:48Z
dc.identifierWILDNER, Letícia Muraro et al . Mycobacteria mobility shift assay: a method for the rapid identification of Mycobacterium tuberculosis and nontuberculous mycobacteria.Mem. Inst. Oswaldo Cruz, Rio de Janeiro , v. 109, n. 3, May 2014
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/9549
dc.identifier10.1590/0074-0276130458.
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886793
dc.descriptionCNPq, LMW and MLB
dc.descriptionThe identification of mycobacteria is essential because tuberculosis (TB) and mycobacteriosis are clinically indistinguishable and require different therapeutic regimens. The traditional phenotypic method is time consuming and may last up to 60 days. Indeed, rapid, affordable, specific and easy-to-perform identification methods are needed. We have previously described a polymerase chain reaction-based method called a mycobacteria mobility shift assay (MMSA) that was designed for Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) species identification. The aim of this study was to assess the MMSA for the identification of MTC and NTM clinical isolates and to compare its performance with that of the PRA-hsp65 method. A total of 204 clinical isolates (102 NTM and 102 MTC) were identified by the MMSA and PRA-hsp65. For isolates for which these methods gave discordant results, definitive species identification was obtained by sequencing fragments of the 16S rRNA and hsp65 genes. Both methods correctly identified all MTC isolates. Among the NTM isolates, the MMSA alone assigned 94 (92.2%) to a complex or species, whereas the PRA-hsp65 method assigned 100% to a species. A 91.5% agreement was observed for the 94 NTM isolates identified by both methods. The MMSA provided correct identification for 96.8% of the NTM isolates compared with 94.7% for PRA-hsp65. The MMSA is a suitable auxiliary method for routine use for the rapid identification of mycobacteria
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz
dc.rightsopen access
dc.subjectnontuberculous mycobacteria
dc.subjectmycobacteria mobility shift assay
dc.subjectmycobacterial identification
dc.titleMycobacteria mobility shift assay: a method for the rapid identification of Mycobacterium tuberculosis and nontuberculous mycobacteria
dc.typeArticle


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