Osteopontin-c isoform levels are associated with SR and hnRNP differential expression in ovarian cancer cell lines

dc.creatorMarques, Durval Santos
dc.creatorGrativol, Jessica
dc.creatorPeres, Rodrigo Alves da Silva
dc.creatorMatos, Aline da Rocha
dc.creatorGimba, Etel Rodrigues Pereira
dc.date2017-11-28T15:09:00Z
dc.date2017-11-28T15:09:00Z
dc.date2017
dc.date.accessioned2023-09-26T23:05:45Z
dc.date.available2023-09-26T23:05:45Z
dc.identifierMARQUES, Durval Santos; et al. Osteopontin-c isoform levels are associated with SR and hnRNP differential expression in ovarian cancer cell lines. Tumor Biology, p.1-8, Sept. 2017.
dc.identifier1010-4283
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23425
dc.identifier10.1177/1010428317725442
dc.identifier1423-0380
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886784
dc.descriptionOsteopontin-c splicing isoform activates ovarian cancer progression features. Imbalanced expression of splicing factors from serine/arginine -rich and heterogeneous ribonucleoproteins families has been correlated with the generation of oncogenic splicing isoforms. Our goal was to investigate whether there is any association between the transcriptional patterns of these splicing factors in ovarian cells and osteopontin-c expression levels. We also aimed to investigate the occurrence of these splicing factors binding sites inside osteopontin exon 4 and adjacent introns. To test associations between osteopontin-c and splicing factors expression patterns, we used an in vitro model in which OVCAR-3 cells overexpressing osteopontin-c (OVCAR-3/OPNc++) presented higher transcriptional levels of osteopontin-c than two other ovarian carcinoma cells (TOV-112D, SKOV-3) and ovarian non-tumoral cell lines (IOSE 364 and IOSE 385). The transcriptional levels of osteopontin-c, serine/arginine-rich, and hnRNP factors were evaluated using real-time polymerase chain reaction. Human Splice Finder software was used to search for putative splicing factor binding sites in osteopontin genomic regions. OVCAR-3/OPNc++ cells presented higher transcriptional levels of hnRNP than serine/arginine-rich when compared to TOV-112D, SKOV-3, and IOSE cells. TOV-112D and SKOV-3 cells also overexpressed hnRNP in relation to serine/arginine-rich transcripts. Putative binding sites for these splicing factors have been predicted on osteopontin exon 4 and their upstream and downstream intronic regions. Our data showed that higher osteopontin-c expression levels are associated with a predominance of hnRNP in relation to serine/arginine-rich transcripts and that osteopontin exon 4 and adjacent intronic sequences contain predicted binding sites for some of these tested splicing factors. In conclusion, differential expression of these splicing factors in ovarian cancer cells could be one of the putative mechanisms leading to aberrant splicing of the osteopontin primary transcript. Future work, aiming to control ovarian cancer progression by downregulating osteopontin-c levels, could include strategies that also regulate heterogeneous ribonucleoproteins and serine/arginine-rich expression levels in order to modulate osteopontin splicing.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer Verlag (Germany)
dc.rightsrestricted access
dc.subjectArginina
dc.subjectProcessamento
dc.subjectribonucleoproteínas heterogêneas
dc.subjectCâncer de ovário
dc.subjectOvarian carcinoma
dc.subjectosteopontin-c
dc.subjectsplicing
dc.subjectsplicing factor regulators
dc.subjecterine/arginine-rich
dc.subjectheterogeneous ribonucleoproteins
dc.titleOsteopontin-c isoform levels are associated with SR and hnRNP differential expression in ovarian cancer cell lines
dc.typeArticle


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