Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome

dc.creatorNascimento, Eduardo J. M.
dc.creatorBraga-Neto, Ulisses
dc.creatorCalzavara-Silva, Carlos E.
dc.creatorGomes, Ana L. V.
dc.creatorAbath, Frederico Guilherme Coutinho
dc.creatorBrito, Carlos A. A.
dc.creatorCordeiro, Marli T.
dc.creatorSilva, Ana M.
dc.creatorMagalhães, Cecilia
dc.creatorAndrade, Raoni
dc.creatorGil, Laura H. V. G.
dc.creatorMarques, Ernesto T. A.
dc.date2018-06-14T11:53:13Z
dc.date2018-06-14T11:53:13Z
dc.date2009
dc.date.accessioned2023-09-26T23:05:25Z
dc.date.available2023-09-26T23:05:25Z
dc.identifierNASCIMENTO, E. J. M. et al. Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome. PloS One, v. 4, n. 11, p. 1-16, 19 Nov. 2009.
dc.identifier1932-6203
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26884
dc.identifier10.1371/journal.pone.0007892
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886719
dc.descriptionBackground: We report the detailed development of biomarkers to predict the clinical outcome under dengue infection. Transcriptional signatures from purified peripheral blood mononuclear cells were derived from whole-genome geneexpression microarray data, validated by quantitative PCR and tested in independent samples. Methodology/Principal Findings: The study was performed on patients of a well-characterized dengue cohort from Recife, Brazil. The samples analyzed were collected prospectively from acute febrile dengue patients who evolved with different degrees of disease severity: classic dengue fever or dengue hemorrhagic fever (DHF) samples were compared with similar samples from other non-dengue febrile illnesses. The DHF samples were collected 2–3 days before the presentation of the plasma leakage symptoms. Differentially-expressed genes were selected by univariate statistical tests as well as multivariate classification techniques. The results showed that at early stages of dengue infection, the genes involved in effector mechanisms of innate immune response presented a weaker activation on patients who later developed hemorrhagic fever, whereas the genes involved in apoptosis were expressed in higher levels. Conclusions/Significance: Some of the gene expression signatures displayed estimated accuracy rates of more than 95%, indicating that expression profiling with these signatures may provide a useful means of DHF prognosis at early stages of infection.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectDengue
dc.subjectPCR
dc.subjectDengue febril aguda
dc.subjectDengue
dc.subjectPCR
dc.subjectAcute febrile dengue
dc.subjectAdulto
dc.subjectIdoso, 80 anos ou mais
dc.subjectApoptose
dc.subjectBiomarcadores / metabolismo
dc.subjectBrasil
dc.subjectEstudos de coorte
dc.subjectDengue / diagnóstico
dc.subjectDengue / genética
dc.subjectDengue / metabolismo
dc.subjectVírus da Dengue / genética
dc.subjectVírus da Dengue / metabolismo
dc.subjectFêmea
dc.subjectFebre / diagnóstico
dc.subjectFebre / metabolismo
dc.subjectRegulamento de Expressão Gênica
dc.subjectHumanos
dc.subjectMasculino
dc.subjectMeia idade
dc.subjectAnálise multivariada
dc.subjectAnálise de Sequência de Sequência de Oligonucleótidos
dc.subjectResultado do tratamento
dc.titleGene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome
dc.typeArticle


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