Trypanosoma cruzi immunoproteome: Calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy

dc.creatorCaminha, Marcelle A.
dc.creatorLorena, Virginia Maria B. de
dc.creatorOliveira Júnior, Wilson de
dc.creatorPerales, Jonas
dc.creatorCarvalho, Paulo C.
dc.creatorLima, Diogo B.
dc.creatorCavalcanti, Maria da Glória A. M.
dc.creatorMartins, Sílvia M.
dc.creatorValente, Richard H.
dc.creatorMenna-Barreto, Rubem F.S.
dc.date2019-09-17T16:04:28Z
dc.date2019-09-17T16:04:28Z
dc.date2019
dc.date.accessioned2023-09-26T23:05:15Z
dc.date.available2023-09-26T23:05:15Z
dc.identifierCAMINHA, Marcelle A. et al. Trypanosoma cruzi immunoproteome: Calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy. Journal of Proteomics, v. 194, p. 179-190, 2019.
dc.identifier1874-3919
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/35642
dc.identifier10.1016/j.jprot.2018.11.019
dc.identifier1876-7737
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886684
dc.descriptionChagas disease, caused by the protozoan Trypanosoma cruzi, affects millions of people worldwide, especially in Latin America. Approximately 30% of the cases evolve to the chronic symptomatic stage due to cardiac and/or digestive damage, generally accompanied by nervous system impairment. Given the higher frequency and severity of clinical manifestations related to cardiac tissue lesion, the goal of this study was the identification of proteins associated with the disease progression towards its cardiac form. Thus, T. cruzi bloodstream trypomastigotes proteins were submitted to immunoprecipitation using antibodies from patients with the asymptomatic or cardiac (stages B1 and C) forms of the disease and from healthy donors as control. Immunoreactive proteins were identified and quantified based on mass spectrometry analysis and shifts in the recognition profile were further evaluated. Compared to asymptomatic samples, IgG from stage C patients predominantly detected the I/6 autoantigen, whereas IgG from B1 patients resulted in higher yield of dihydrolipoamide acetyltransferase precursor, calpain cysteine peptidase, and two variants of CAP5.5. In this work, CAP5.5 recognition by serum immunoglobulin from patients with early cardiomyopathy generated a 23-fold abundance variation when compared to samples from asymptomatic patients, highlighting the participation of this protein in cardiac form progression of the disease. SIGNIFICANCE: While T. cruzi has become the major cause of infectious cardiomyopathy in Latin America, research groups have been struggling to find alternative treatment, vaccine candidates, and improved diagnostic tests. In addition, the absence of adequate biomarkers to assess cure and progression of disease is a major setback for clinical trials and patients monitoring. Therefore, our findings may contribute to a better understanding of T. cruzi pathogenesis and evaluation of suitable candidates for vaccine and diagnostic tests, besides the clinical applicability of the potential biomarkers for patient follow-up and prognosis. Finally, the identification of T. cruzi proteins recognized by IgG from healthy donors may contribute for the understanding and discovery of epitope conservation among a broad range of pathogens.
dc.description2025-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectDoença de Chagas
dc.subjectBiomarcadores
dc.subjectEspectometria de massa
dc.subjectCardiomiopatia
dc.subjectTrypanosoma cruzi
dc.subjectChagas Disease
dc.subjectCardiomyopathy
dc.subjectBiomarkers
dc.subjectMass spectrometry
dc.subjectCAP5.5
dc.titleTrypanosoma cruzi immunoproteome: Calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy
dc.typeArticle


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