Accelerating the morphogenetic cycle of the viral vector Aedes aegypti larvae for faster larvicidal bioaasys

dc.creatorFontana, José Domingues
dc.creatorFerreira, Rafael Lopes
dc.creatorZuccolotto, Tatiana
dc.creatorDalagassa, Cibelle de Borba
dc.creatorWielewski, Leonardo Pellizzari
dc.creatorChalcoski, Barbara Maria Santano
dc.creatorSilva, Mario Antonio Navarro da
dc.creatorRichardi, Vinicius Sobrinho
dc.creatorGolart, Jonas
dc.creatorRodovalho, Cynara de Melo
dc.date2020-12-16T19:11:56Z
dc.date2020-12-16T19:11:56Z
dc.date2020
dc.date.accessioned2023-09-26T23:05:03Z
dc.date.available2023-09-26T23:05:03Z
dc.identifierFONTANA, José Domingos et al. Accelerating the Morphogenetic Cycle of the Viral Vector Aedes aegypti Larvae for Faster Larvicidal Bioassays. Biomed Res Int., Vol. 2020, ID 7405421, 9p, Aug. 2020.
dc.identifier2314-6133
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44944
dc.identifier2314-6141
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886646
dc.descriptionBioMed Research International [English) - Open Access pathways permitted by this journal's policy are listed below by article version. Click on a pathway for a more detailed view Published Version NoneCC BYPMC Any Website, Journal Website OA PublishingThis pathway includes Open Access publishing Embargo No Embargo
dc.descriptionAny bioassay to test new chemically synthesized larvicides or phytolarvicides against Culicidae and more harmful mosquito species, such as Aedes aegypti and Aedes albopictus, which specifically transmit dengue, yellow fever, chikungunya viral fevers as well as Zika virus, or Anopheles gambiae, a vector for malaria and philariasis, requires thousands of well-developed larvae, preferably at the fourth instar stage. The natural morphogenetic cycle of Aedes spp., in the field or in the laboratory, may extend to 19 days at room temperature (e.g., 25°C) from the first permanent contact between viable eggs and water and the last stage of larval growth or metamorphosis into flying adults. Thus, accelerated sequential molting is desirable for swifter bioassays of larvicides. We achieved this goal in Aedes aegypti with very limited strategic and low-cost additions to food, such as coconut water, milk or its casein, yeast extract, and to a lesser extent, glycerol. The naturally rich coconut water was excellent for quickly attaining the population of instar IV larvae, the most advanced one before pupation, saving about a week, for subsequent larvicidal bioassays. Diluted milk, as another food source, allowed an even faster final ecdysis and adults are useful for mosquito taxonomical purpose.
dc.formatapplication/pdf
dc.languageeng
dc.publisherHindawi
dc.rightsopen access
dc.subjectAcelerando
dc.subjectCiclo morfogenético
dc.subjectLarvas
dc.subjectVetor viral
dc.subjectAedes aegypti
dc.subjectBioensaios larvicidas
dc.subjectAedes aegypti Larvae
dc.subjectMorphogenetic Cycle
dc.subjectLarvicidal Bioassays
dc.subjectAccelerating
dc.subjectViral Vector
dc.titleAccelerating the morphogenetic cycle of the viral vector Aedes aegypti larvae for faster larvicidal bioaasys
dc.typeArticle


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