Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes

dc.creatorBatista, Cassiano Martin
dc.creatorSaad, Felipe
dc.creatorCeccoti, Stephane Pini Costa
dc.creatorEger, Iriane
dc.creatorSoares, Maurilio José
dc.date2018-09-20T15:36:58Z
dc.date2018-09-20T15:36:58Z
dc.date2018
dc.date.accessioned2023-09-26T23:04:51Z
dc.date.available2023-09-26T23:04:51Z
dc.identifierBATISTA, Cassiano Martin et al. Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v.113, n.8, 2018.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28942
dc.identifier10.1590/0074-02760180086
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886606
dc.descriptionDynamic S-palmitoylation of proteins is the addition of palmitic acid by zDHHC palmitoyl transferases (PATs) and depalmitoylation by palmitoyl protein thioesterases (PPTs). A putative PAT (TcPAT1) has been previously identified in Trypanosoma cruzi, the etiological agent of Chagas disease. Here we analyse other 14 putative TcPATs and 2 PPTs in the parasite genome. T. cruzi cell lines expressing TcPATs and TcPPTs plus a FLAG tag at the C terminus were produced for most enzymes, with positive detection by indirect immunofluorescence. Overexpressed TcPATs were mostly found as single spots at the parasite anterior end, while the TcPPTs were dispersed throughout the parasite body.
dc.formatapplication/pdf
dc.languagepor
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectTrypanosomatina
dc.subjectLipoylation
dc.subjectPalmitic Acid
dc.subjectGene Expression
dc.subjectLipoilación
dc.subjectÁcido Palmítico
dc.subjectExpresión Génica
dc.subjectLipoilação
dc.subjectÁcido Palmítico
dc.subjectExpressão Gênica
dc.titleSubcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes
dc.typeArticle


Este ítem pertenece a la siguiente institución