Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma cruzi

dc.creatorAdesse, Daniel
dc.creatorIacobas, Dumitru A.
dc.creatorIacobas, Sanda
dc.creatorGarzoni, Luciana R.
dc.creatorMeirelles, Maria de Nazareth
dc.creatorTanowitz, Herbert B.
dc.creatorSpray, David C.
dc.date2018-08-08T12:36:37Z
dc.date2018-08-08T12:36:37Z
dc.date2010
dc.date.accessioned2023-09-26T23:03:45Z
dc.date.available2023-09-26T23:03:45Z
dc.identifierADESSE, Daniel; et al. Transcriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma Cruzi. Am. J. Trop. Med. Hyg., v.82, n.5, p.846–854, 2010.
dc.identifier0002-9637
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28000
dc.identifier10.4269/ajtmh.2010.09-0399
dc.identifier1476-1645
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886402
dc.descriptionWe examined the extent to which different Trypanosoma cruzi strains induce transcriptomic changes in cultured L(6)E(9) myoblasts 72 hours after infection with Brazil (TC I), Y (TC II), CL (TC II), and Tulahuen (TC II) strains. Expression of 6,289 distinct, fully annotated unigenes was quantified with 27,000 rat oligonucleotide arrays in each of the four replicas of all control and infected RNA samples. Considering changes greater than 1.5-fold and P values < 0.05, the Tulahuen strain was the most disruptive to host transcriptome (17% significantly altered genes), whereas the Y strain altered only 6% of the genes. The significantly altered genes in the infected cells were largely different among the strains, and only 21 genes were similarly changed by all four strains. However, myoblasts infected with different strains showed proportional overall gene-expression alterations. These results indicate that infection with different parasite strains modulates similar but not identical pathways in the host cells.
dc.formatapplication/pdf
dc.languageeng
dc.publisherThe American Society of Tropical Medicine and Hygiene
dc.rightsopen access
dc.subjectTrypanosoma Cruzi
dc.subjectAssinaturas transcriptômicas
dc.subjectLinhagem mioblástica
dc.subjectCepas
dc.subjectInfecção
dc.subjectBrasil
dc.subjectTrypanosoma Cruzi
dc.subjectTranscriptomic Signatures
dc.subjectMyoblast Cell Line
dc.subjectFour Distinct Strains
dc.subjectInfection
dc.subjectBrazil
dc.titleTranscriptomic signatures of alterations in a myoblast cell line infected with four distinct strains of Trypanosoma cruzi
dc.typeArticle


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