dc.creatorGenteluci, Gabrielle Limeira
dc.creatorGomes, Daniela Betzler Cardoso
dc.creatorPereira, Daniella Cristina Rodrigues
dc.creatorNeves, Marta de Campos
dc.creatorSouza, Maria José de
dc.creatorRangel, Karyne
dc.creatorVillas Bôas, Maria Helena Simões
dc.date2020-11-12T20:57:37Z
dc.date2020-11-12T20:57:37Z
dc.date2020
dc.date.accessioned2023-09-26T23:03:16Z
dc.date.available2023-09-26T23:03:16Z
dc.identifierGENTELUCI, Gabrielle Limeira et al. Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells. Brazilian Journal of Microbiology, São Paulo, v. 51, p. 657–664, mar. 2020.
dc.identifier1517-8382
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44390
dc.identifier10.1007/s42770-020-00252-x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886306
dc.descriptionDaniella Pereira - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento do vinculo com a Fiocruz Brasília.
dc.descriptionAcinetobacter baumannii has been associated with antimicrobial resistance and ability to form biofilms. Furthermore, its adherence to host cells is an important factor to the colonization process. Therefore, this study intended to identify some virulence factors that can explain the success of A. baumannii in causing nosocomial infections. We studied 92 A. baumannii isolates collected from hospitals in Rio de Janeiro, Brazil. Isolates were identified and the susceptibility to antimicrobials was determined. Oxacilinase type β-lactamase encoding genes were amplified by polymerase chain reaction, and genetic diversity was investigated by pulsed-field gel electrophoresis (PFGE). In addition, biofilm formation on polystyrene plates using crystal violet staining was quantified, and adherence to human cell lines was evaluated. Eighty-six isolates were multidrug-resistant, of which 93% were carbapenem-resistant. All isolates had the blaOXA-51 gene and 94% had the blaOXA-23 gene, other searched blaOXA genes were not detected. PFGE typing showed two predominant clones, and biofilm production was observed in 79%of isolates. A. baumannii isolates adhered better to HEp-2 cell compared with A-549 cell. Clones A, B, E, and F showed a significantly increased adherence to HEp-2 compared with adherence to A-549 cell. Our findings revealed that A. baumannii isolates had high frequencies of resistance to antimicrobial agents, ability to form biofilm, and capacity to adhere to HEp-2 cells.
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociedade Brasileira de Microbiologia
dc.rightsopen access
dc.subjectAcinetobacter baumannii
dc.subjectDrug Resistance, Microbial
dc.subjectBiofilms
dc.subjectAcinetobacter baumannii
dc.subjectAntimicrobial resistance
dc.subjectPCR
dc.subjectPFGE
dc.subjectBiofilm
dc.subjectAdherence to cell
dc.subjectAcinetobacter baumannii
dc.subjectResistência Microbiana a Medicamentos
dc.subjectBiofilmes
dc.titleMultidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells
dc.typeArticle


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