Detection of Plasmodium in faeces of the New World primate Alouatta clamitans.

dc.creatorAssis, Gabriela Maíra Pereira de
dc.creatorAlvarenga, Denise Anete Madureira de
dc.creatorCosta, Daniela Camargos
dc.creatorSouza Junior, Júlio César de
dc.creatorHirano, Zelinda Maria Braga
dc.creatorKano, Flora Satiko
dc.creatorSousa, Taís Nóbrega de
dc.creatorBrito, Cristiana Ferreira Alves de
dc.date2017-07-12T17:30:23Z
dc.date2017-07-12T17:30:23Z
dc.date2016
dc.date.accessioned2023-09-26T23:03:02Z
dc.date.available2023-09-26T23:03:02Z
dc.identifierASSIS, Gabriela Maíra Pereira de et al. Detection of Plasmodium in faeces of the New World primate Alouatta clamitans. Mem. Inst. Oswaldo Cruz, v. 111, n. 9, p. 570-576, 2016.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/20058
dc.identifier10.1590/0074-02760160222
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886259
dc.descriptionPlasmodium falciparum and Plasmodium vivax have evolved with host switches between non-human primates (NHPs) and humans. Studies on the infection dynamics of Plasmodium species in NHPs will improve our understanding of the evolution of these parasites; however, such studies are hampered by the difficulty of handling animals in the field. The aim of this study was to detect genomic DNA of Plasmodium species from the faeces of New World monkeys. Faecal samples from 23 Alouatta clamitans from the Centre for Biological Research of Indaial (Santa Catarina, Brazil) were collected. Extracted DNA from faecal samples was used for molecular diagnosis of malaria by nested polymerase chain reaction. One natural infection with Plasmodium simium was identified by amplification of DNA extracted from the faeces of A. clamitans. Extracted DNA from a captive NHP was also used for parasite genotyping. The detection limit of the technique was evaluated in vitro using an artificial mixture of cultured P. falciparum in NHP faeces and determined to be 6.5 parasites/µL. Faecal samples of New World primates can be used to detect malaria infections in field surveys and also to monitor the genetic variability of parasites and dynamics of infection.
dc.formatapplication/pdf
dc.languageeng
dc.publisherInstituto Oswaldo Cruz
dc.rightsopen access
dc.subjectPlasmodium vivax
dc.subjectNew World primates
dc.subjectAlouatta clamitans
dc.subjectsimian malaria
dc.subjectPlasmodium simium
dc.titleDetection of Plasmodium in faeces of the New World primate Alouatta clamitans.
dc.typeArticle


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