The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells

dc.creatorFracaro, Letícia
dc.creatorSenegaglia, Alexandra C.
dc.creatorHerai, Roberto H.
dc.creatorLeitolis, Amanda
dc.creatorBoldrini-Leite, Lidiane M.
dc.creatorRebelatto, Carmen L. K.
dc.creatorTravers, Paul J.
dc.creatorBrofman, Paulo R S
dc.creatorDominguez, Alejandro Correa
dc.date2020-05-12T19:32:40Z
dc.date2020-05-12T19:32:40Z
dc.date2020
dc.date.accessioned2023-09-26T23:02:46Z
dc.date.available2023-09-26T23:02:46Z
dc.identifierFRACARO, Leticia et al. The Expression Profile of Dental Pulp-Derived Stromal Cells Supports Their Limited Capacity to Differentiate into Adipogenic Cells. International Journal of Molecular Sciences. V.21, n.2753, p. 1-17, 2020.
dc.identifier1422-0067
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/41209
dc.identifier10.3390/ijms21082753
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886209
dc.descriptionMesenchymal stromal cells (MSCs) can self-renew, differentiate into specialised cells and have different embryonic origins-ectodermal for dental pulp-derived MSCs (DPSCs) and mesodermal for adipose tissue-derived MSCs (ADSCs). Data on DPSCs adipogenic differentiation potential and timing vary, and the lack of molecular and genetic information prompted us to gain a better understanding of DPSCs adipogenic differentiation potential and gene expression profile. While DPSCs differentiated readily along osteogenic and chondrogenic pathways, after 21 days in two different types of adipogenic induction media, DPSCs cultures did not contain lipid vacuoles and had low expression levels of the adipogenic genes proliferator-activated receptor gamma (PPARG), lipoprotein lipase (LPL) and CCAAT/enhancer-binding protein alpha (CEBPA). To better understand this limitation in adipogenesis, transcriptome analysis in undifferentiated DPSCs was carried out, with the ADSC transcriptome used as a positive control. In total, 14,871 transcripts were common to DPSCs and ADSCs, some were unique (DPSCs: 471, ADSCs: 1032), and 510 were differentially expressed genes. Detailed analyses of overrepresented transcripts showed that DPSCs express genes that inhibit adipogenic differentiation, revealing the possible mechanism for their limited adipogenesis.
dc.formatapplication/pdf
dc.languagepor
dc.publisherMDPI
dc.rightsopen access
dc.subjectDPSCs
dc.subjectADSCs
dc.subjectMesenchymal Stem Cells
dc.subjectDental Pulp
dc.subjectAdipose Tissue
dc.subjectAdipogenesis
dc.subjectTranscription Factors
dc.subjectWnt Signaling Pathway
dc.subjectCélulas Madre Mesenquimatosas
dc.subjectPulpa Dental
dc.subjectTejido Adiposo
dc.subjectAdipogénesis
dc.subjectFactores de Transcripción
dc.subjectVía de Señalización Wnt
dc.subjectCélulas-Tronco Mesenquimais
dc.subjectPolpa Dentária
dc.subjectTecido Adiposo
dc.subjectAdipogenia
dc.subjectFatores de Transcrição
dc.subjectVia de Sinalização Wnt
dc.titleThe expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells
dc.typeArticle


Este ítem pertenece a la siguiente institución