Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types

dc.creatorAlmeida-Silva, Fernando
dc.creatorBarbedo, Leonardo Silva
dc.creatorTaylor, Maria Lucia
dc.creatorMuniz, Mauro de Medeiros
dc.creatorGuimarães, Allan Jefferson
dc.creatorZancopé-Oliveira, Rosely Maria
dc.date2019-01-10T13:36:37Z
dc.date2019-01-10T13:36:37Z
dc.date2018
dc.date.accessioned2023-09-26T23:02:40Z
dc.date.available2023-09-26T23:02:40Z
dc.identifierALMEIDA-SILVA, Fernanda et al. Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types. Memórias do Instiuto Oswaldo Cruz, v. 113, n. 10, p. 1-4, 2018.
dc.identifier1678-8060
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30959
dc.identifier10.1590/0074-02760180340
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886190
dc.descriptionHistoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, a heterothallic ascomycete. The sexual reproduction of this fungus is regulated by the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified by uniplex polymerase chain reaction (PCR). This study aimed to optimise single-step multiplex PCR for the accurate detection of the distinct mating types of H. capsulatum. Among the 26 isolates tested, 20 had MAT1-1 genotype, while six showed MAT1-2 genotype, in agreement with the uniplex PCR results. These results suggest that multiplex PCR is a fast and specific tool for screening H. capsulatum mating types.
dc.formatapplication/pdf
dc.languageeng
dc.publisherInstituto Oswaldo Cruz
dc.rightsopen access
dc.subjectMating type
dc.subjectMAT1
dc.subjectHistoplasma capsulatum
dc.subjectMultiplex PCR
dc.titleMultiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types
dc.typeArticle


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