Transcriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humans

dc.creatorMaretti-Mira, Ana Claudia
dc.creatorBittner, Jaime
dc.creatorOliveira Neto, Manoel Paes
dc.creatorLiu, Minghsun
dc.creatorKang, Dezhi
dc.creatorLi, Huiying
dc.creatorPirmez, Claude
dc.creatorCraft, Noah
dc.date2017-05-16T11:26:43Z
dc.date2017-05-16T11:26:43Z
dc.date2012
dc.date.accessioned2023-09-26T23:01:52Z
dc.date.available2023-09-26T23:01:52Z
dc.identifierMARRETI-MIRA, Ana Claudia et al. Transcriptome Patterns from Primary Cutaneous Leishmania braziliensis Infections Associate with Eventual Development of Mucosal Disease in Humans. PLoS Neglected Tropical Diseases, v. 6, n. 9, p. 1-11, Sept. 2012.
dc.identifier1935-2727
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/18853
dc.identifier10.1371/journal.pntd.0001816
dc.identifier1935-2735
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886034
dc.descriptionIntroduction: Localized Cutaneous Leishmaniasis (LCL) and Mucosal Leishmaniasis (ML) are two extreme clinical forms of American Tegumentary Leishmaniasis that usually begin as solitary primary cutaneous lesions. Host and parasite factors that influence the progression of LCL to ML are not completely understood. In this manuscript, we compare the gene expression profiles of primary cutaneous lesions from patients who eventually developed ML to those that did not. Methods: Using RNA-seq, we analyzed both the human and Leishmania transcriptomes in primary cutaneous lesions. Results: Limited number of reads mapping to Leishmania transcripts were obtained. For human transcripts, compared to ML patients, lesions from LCL patients displayed a general multi-polarization of the adaptive immune response and showed upregulation of genes involved in chemoattraction of innate immune cells and in antigen presentation. We also identified a potential transcriptional signature in the primary lesions that may predict long-term disease outcome. Conclusions: We were able to simultaneously sequence both human and Leishmania mRNA transcripts in primary cutaneous leishmaniasis lesions. Our results suggest an intrinsic difference in the immune capacity of LCL and ML patients. The findings correlate the complete cure of L. braziliensis infection with a controlled inflammatory response and a balanced activation of innate and adaptive immunity.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectLeishmaniose cutânea
dc.subjectDoença das mucosas
dc.subjectHumanos
dc.subjectLeishmaniose mucosa
dc.subjectMucosal Leishmaniasis
dc.subjectMucosal Disease
dc.subjectCutaneous Leishmania braziliensis
dc.subjectHumans
dc.titleTranscriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humans
dc.typeArticle


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