Ultrastructural and cytochemical aspects of Schistosoma mansoni cercaria

dc.creatorCavalcanti, M. G. S.
dc.creatorAraújo, H. R. C.
dc.creatorPaiva, M. H. S.
dc.creatorSilva, G. M.
dc.creatorBarbosa, C. C. G. S.
dc.creatorSilva, L. F.
dc.creatorBrayner, F. A.
dc.creatorAlves, L. C.
dc.date2018-07-12T13:55:46Z
dc.date2018-07-12T13:55:46Z
dc.date2009
dc.date.accessioned2023-09-26T23:01:08Z
dc.date.available2023-09-26T23:01:08Z
dc.identifierCAVALCANTI, M. G. S. et al. Ultrastructural and cytochemical aspects of Schistosoma mansoni cercaria. Micron (Oxford, England: 1993), v. 40, n. 3, p. 394–400, abr. 2009.
dc.identifier0968-4328
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/27506
dc.identifier10.1016/j.micron.2008.09.006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8885897
dc.descriptionAn alternative to identify the critical processes necessary to the parasite establishment of the host is to focus on the evolutionary stage responsible for the primary invasion, i.e. the infection structure. The objective of this study was to ultrastructurally characterize Schistosoma mansoni cercariae, using cytochemical techniques. In order to identify basic proteins, techniques such as ethanolic phosphotungstic acid (EPTA) and ammoniacal silver staining were used. Calcium sites location was achieved using the Hepler technique and to evidence anionic groups, we used cationic ferritin particles and enzyme treatment with trypsin Vibrio cholerae, chondroitinase and neuraminidase. The EPTA technique highlighted the presence of basic tegument proteins, nucleus and nucleolus from subtegumental cells, inclusion bodies and preacetabular glands. After using ammoniacal silver, we observed a strong staining in all infective larvae, particularly in the nuclei of muscle cells, circular muscle tissue and preacetabular glands. Calcium site locations were shown to be uniform, thereby limiting the inner spaces of the larvae, especially muscle cells. Samples treated with cationized ferritin particles presented strong staining at the cuticular level. Neuraminidase treatment did not alter the stained shape of such particles on the trematode surface. However, trypsin or chondroitinase treatment resulted in absence of staining on the larval surface. This information on the biochemical composition of the infecting S. mansoni larvae provides data for a better understanding of the biology of this parasite and background on the intriguing parasite-host relationship.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectSchistosoma mansoni cercaria
dc.subjectCitoquímica ultraestrutural
dc.subjectSchistosoma mansoni cercaria
dc.subjectUltrastructural cytochemical
dc.subjectAnimais
dc.subjectMembrana de Porão / química
dc.subjectMembrana de Porão / ultraestrutura
dc.subjectCálcio / Química
dc.subjectCondroitinases e Condroitinas Liases / metabolismo
dc.subjectProteínas de Helmintos / química
dc.subjectProteínas de Helmintos / ultraestrutura
dc.subjectInterações Hospedeiro-Parasita / fisiologia
dc.subjectHumanos
dc.subjectÓrgãos de Inclusão / química
dc.subjectCorpos de Inclusão / ultraestrutura
dc.subjectLarva / química
dc.subjectLarva / enzimologia
dc.subjectLarva / ultraestrutura
dc.subjectMicroscopia Eletrônica, Transmissão
dc.subjectMúsculos / química
dc.subjectMúsculos / ultraestrutura
dc.subjectNeuraminidase / metabolismo
dc.subjectSchistosoma mansoni / química
dc.subjectSchistosoma mansoni / metabolismo
dc.subjectSchistosoma mansoni / ultraestrutura
dc.subjectEsquistossomose / parasitologia
dc.subjectTripsina / metabolismo
dc.titleUltrastructural and cytochemical aspects of Schistosoma mansoni cercaria
dc.typeArticle


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