Identification and characterization of microsatellite markers for population genetic studies of Panstrongylus megistus (Burmeister, 1835) (Triatominae: Reduviidae)

dc.creatorFerreira, Flávio Campos
dc.creatorGonçalves, Leilane Oliveira
dc.creatorRuiz, Jeronimo Conceição
dc.creatorKoerich, Leonardo Barbosa
dc.creatorPais, Fabiano Sviatopolk Mirsky
dc.creatorDiotaiuti, Liléia Gonçalves
dc.creatorBelisário, Carlota Josefovicz
dc.date2022-03-04T19:55:36Z
dc.date2022-03-04T19:55:36Z
dc.date2021
dc.date.accessioned2023-09-26T23:00:33Z
dc.date.available2023-09-26T23:00:33Z
dc.identifierFERREIRA, Flávio Campos et al. Identification and characterization of microsatellite markers for population genetic studies of Panstrongylus megistus (Burmeister, 1835) (Triatominae: Reduviidae). Parasit Vectors, v. 14, n .1, p. 1-8, 2021.
dc.identifier1756-3305
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/51563
dc.identifier10.1186/s13071-021-04771-w
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8885800
dc.descriptionBackground: Panstrongylus megistus is the most important vector of Chagas disease in Brazil. Studies show that the principal factor hindering the control of triatomines is reinfestation of houses previously treated with insecticides. Studies at the microgeographic level are therefore necessary to better understand these events. However, an efficient molecular marker is not yet available for carrying out such analyses in this species. The aim of the present study was to identify and characterize microsatellite loci for future population genetic studies of P. megistus. Methods: This study work consisted of five stages: (i) sequencing of genomic DNA; (ii) assembly and selection of contigs containing microsatellites; (iii) validation of amplification and evaluation of polymorphic loci; (iv) standardization of the polymorphic loci; and (v) verification of cross-amplification with other triatomine species. Results: Sequencing of males and females generated 7,908,463 contigs with a total length of 2,043,422,613 bp. A total of 2,043,690 regions with microsatellites in 1,441,091 contigs were obtained, with mononucleotide repeats being the most abundant class. From a panel of 96 loci it was possible to visualize polymorphisms in 64.55% of the loci. Of the 20 loci genotyped, the number of alleles varied from two to nine with an average of 4.9. Cross-amplification with other species of triatomines was observed in 13 of the loci. Conclusions: Due to the high number of alleles encountered, polymorphism and the capacity to amplify from geographically distant populations, the microsatellites described here show promise for utilization in population genetic studies of P. megistus.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBioMed Central
dc.rightsopen access
dc.subjectChagas Disease
dc.subjectMicrosatellites
dc.subjectPanstrongylus megistus
dc.subjectTriatominae
dc.titleIdentification and characterization of microsatellite markers for population genetic studies of Panstrongylus megistus (Burmeister, 1835) (Triatominae: Reduviidae)
dc.typeArticle


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