Pathogen blocking in Wolbachia-infected Aedes aegypti is not affected by Zika and dengue virus co-infection

dc.creatorCaragata, Eric Pearce
dc.creatorRocha, Marcele Neves
dc.creatorPereira, Thiago Nunes
dc.creatorMansur, Simone Brutman
dc.creatorDutra, Heverton Leandro Carneiro
dc.creatorMoreira, Luciano Andrade
dc.date2019-08-26T13:44:59Z
dc.date2019-08-26T13:44:59Z
dc.date2019
dc.date.accessioned2023-09-26T23:00:29Z
dc.date.available2023-09-26T23:00:29Z
dc.identifierCARAGATA, Eric Pearce et al. Pathogen blocking in Wolbachia-infected Aedes aegypti is not affected by Zika and dengue virus co-infection. PLoS Neglected Tropical Diseases, v. 13, n. 5, p. 1-26, 2019.
dc.identifier1935-2735
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/35065
dc.identifier10.1371/journal.pntd.0007443
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8885788
dc.descriptionBackground: Wolbachia’s ability to restrict arbovirus transmission makes it a promising tool to combat mosquito-transmitted diseases. Wolbachia-infected Aedes aegypti are currently being released in locations such as Brazil, which regularly experience concurrent outbreaks of different arboviruses. A. aegypti can become co-infected with, and transmit multiple arboviruses with one bite, which can complicate patient diagnosis and treatment. Methodology/principle findings: Using experimental oral infection of A. aegypti and then RT-qPCR, we examined ZIKV/DENV-1 and ZIKV/DENV-3 co-infection in Wolbachia-infected A. aegypti and observed that Wolbachia-infected mosquitoes experienced lower prevalence of infection and viral load than wildtype mosquitoes, even with an extra infecting virus. Critically, ZIKV/DENV co-infection had no significant impact on Wolbachia’s ability to reduce viral transmission. Wolbachia infection also strongly altered expression levels of key immune genes Defensin C and Transferrin 1, in a virus-dependent manner. Conclusions/significance: Our results suggest that pathogen interference in Wolbachia-infected A. aegypti is not adversely affected by ZIKV/DENV co-infection, which suggests that Wolbachia-infected A. aegypti will likely prove suitable for controlling mosquito-borne diseases in environments with complex patterns of arbovirus transmission.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectMosquitoes
dc.subjectCo-infections
dc.subjectWolbachia
dc.subjectArboviral infections
dc.subjectSaliva
dc.subjectViral pathogens
dc.subjectViral transmission and infection
dc.subjectViral load
dc.titlePathogen blocking in Wolbachia-infected Aedes aegypti is not affected by Zika and dengue virus co-infection
dc.typeArticle


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