Assessment of a quantitative 5' nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

dc.creatorBenevenute, Jyan Lucas
dc.creatorDumler, John Stephen
dc.creatorOgrzewalska, Maria
dc.creatorRoque, André Luiz Rodrigues
dc.creatorMello, Victoria Valente Califre
dc.creatorSousa, Keyla Carstens Marques de
dc.creatorGonçalves, Luiz Ricardo
dc.creatorD'Andrea, Paulo Sérgio
dc.creatorLemos, Elba Regina Sampaio de
dc.creatorMachado, Rosangela Zacarias
dc.creatorAndré, Marcos Rogério
dc.date2017-06-29T16:28:21Z
dc.date2017-06-29T16:28:21Z
dc.date2017
dc.date.accessioned2023-09-26T23:00:28Z
dc.date.available2023-09-26T23:00:28Z
dc.identifierBENEVUTE, Jyan Lucas; et al. Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brasil. Ticks and Tick-borne Diseases, v.8, n.4, p.646-656, June 2017.
dc.identifier1877-959X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/19676
dc.identifier10.1016/j.ttbdis.2017.04.011
dc.identifier1877-9603
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8885783
dc.descriptionNew genotypes of Anaplasmataceae agents have been detected in wild carnivores, birds and deer in Brazil. The present work aimed to investigate the presence of Ehrlichia and Anaplasma species in rodents sampled in Brazil. Additionally, a newly designed quantitative 5' nuclease real-time multiplex PCR for Ehrlichia and Anaplasma spp. detection based on groEL gene amplification was designed, showing high specificity and sensitivity (10 groEL fragment copy/μL). Between 2000 and 2011, different rodent species [n=60] were trapped in 5 Brazilian biomes. Among 458 rodent spleen samples, 0.4% (2/458) and 2.4% (11/458) were positive for Ehrlichia and Anaplasma spp., respectively. Of 458 samples, 2.0% (9/458) and 1.1% (5/458) were positive for Anaplasma sp. and Ehrlichia sp., respectively, using conventional 16S rRNA PCR assays. Maximum Likelihood phylogenetic analyse based on a small region of 16S rRNA genes positioned the Anaplasma genotypes in rodents near Anaplasma phagocytophilum or Anaplasma marginale and Anaplasma odocoilei isolates. Ehrlichia genotypes were closely related to E. canis. There was a low occurrence of Anaplasma and Ehrlichia in wild and synanthropic rodents in Brazil, suggesting the circulation of new genotypes of these agents in rodents in the studied areas.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectRoedores sinatrópicos
dc.subjectEhrlichia
dc.subjectAnaplasma
dc.subjectBrasil
dc.subjectReação em Cadeia da Polimerase
dc.subjectChaperonina 60
dc.subjectEhrlichia
dc.subjectAnaplasma
dc.subjectsynanthropic rodents
dc.subjectBrazil
dc.subjectgenotypes
dc.subjectreal-time polymerase chain reaction
dc.titleAssessment of a quantitative 5' nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil
dc.typeArticle


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