B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis

dc.creatorAssis, Luciana Moura de
dc.creatorSousa, J. R
dc.creatorPinto, N. F. S
dc.creatorSilva, A. A
dc.creatorVaz, Antonio Fernando de Melo
dc.creatorAndrade, Paulo Paes
dc.creatorCarvalho, Edgar Marcelino de
dc.creatorMelo, Marcia Almeida de
dc.date2018-02-20T14:18:03Z
dc.date2018-02-20T14:18:03Z
dc.date2014
dc.date.accessioned2023-09-26T22:59:49Z
dc.date.available2023-09-26T22:59:49Z
dc.identifierASSIS, L. M. et al. B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis. Parasite Immunology, v. 36, p. 313–323, 2014.
dc.identifier0141-9838
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/24882
dc.identifier10.1111/pim.12111
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8885657
dc.descriptionCARVALHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
dc.descriptionNational Institute of Science and Technology of Tropical Diseases (Instituto Nacional de Ci^encia e Tecnologia de Doenc as Tropicais – INCT-DT), MCT/CNPq,
dc.descriptionSerodiagnosis of visceral leishmaniasis is often hindered by cross-reactions to other parasitic diseases. Identifying specific B-cell epitopes in proteins is therefore important for immunodiagnostics, as well as for disease control by vaccines. This study aimed to identify linear and conformational B-cell epitopes and to evaluate the secondary structure of antigen proteins in Leishmania infantum using in silico analysis. Linear epitopes were predicted using the Immune Epitope Database and Analysis Resource (IEDB), BepiPred and BcePred programs. The conformational B-cell epitopes were identified using the CBTOPE server. The combination of the predictions using IEDB, BepiPred and BcePred generated 148 linear epitopes from the calpain-like cysteine peptidase (CP), thiol-dependent reductase 1 (TDR1) and HSP70 proteins. In total, 164 conformational epitopes were predicted, mostly located in the linear epitope region. The predicted epitopes are located in α helix and random coil regions in the thiol-dependent reductase 1 and HSP70 proteins. New linear and conformational B-cell epitopes of L. infantum proteins were identified in silico, and the prediction using various programs ensures greater accuracy of the results, as suggested by confirmation of previously identified HSP70 epitopes.
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.rightsopen access
dc.subjectBioinformática
dc.subjectDiagnóstico
dc.subjectKala-azar
dc.subjectBiologia sintética
dc.subjectVacina
dc.subjectBioinformatics
dc.subjectDiagnosis
dc.subjectKala-azar
dc.subjectSynthetic biology
dc.subjectVaccine
dc.titleB-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis
dc.typeArticle


Este ítem pertenece a la siguiente institución