Anti-genotoxic effect of aqueous extracts of sun mushroom (Agaricus blazei Murill lineage 99/26) in mammalian cells in vitro

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMartins de Oliveira, J.
dc.creatorJordão, B. Q.
dc.creatorRibeiro, Lúcia Regina
dc.creatorFerreira Da Eira, A.
dc.creatorMantovani, M. S.
dc.date2014-05-27T11:20:32Z
dc.date2016-10-25T18:18:05Z
dc.date2014-05-27T11:20:32Z
dc.date2016-10-25T18:18:05Z
dc.date2002-12-01
dc.date.accessioned2017-04-06T01:03:38Z
dc.date.available2017-04-06T01:03:38Z
dc.identifierFood and Chemical Toxicology, v. 40, n. 12, p. 1775-1780, 2002.
dc.identifier0278-6915
dc.identifierhttp://hdl.handle.net/11449/67032
dc.identifierhttp://acervodigital.unesp.br/handle/11449/67032
dc.identifier10.1016/S0278-6915(02)00156-4
dc.identifierWOS:000179599000006
dc.identifier2-s2.0-0036888059
dc.identifierhttp://dx.doi.org/10.1016/S0278-6915(02)00156-4
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/888525
dc.descriptionThe sun mushroom is the popular name for the Agaricus blazei Murill fungus, a mushroom native to south-eastern Brazil, which has been frequently used in popular medicine mainly in the form of tea to treat various ailments (stress, diabetes, etc.). In the present study, the genotoxic and/or anti-genotoxic effects ofA. blazei on mammalian cells in culture was assessed by checking the increase or reduction of micronucleus (MN) frequency and comets. The sun mushroom (lineage 99/26) was used as aqueous extracts prepared (2.5%) at three different temperatures (60, 25 and 4°C). The in vitro micronucleus (MN) test in binucleated cells and comet assay were used in V79 cells cultivated in HAM-F10+DMEM medium (1:1), supplemented with 10% of fetal bovine serum. The experiments were divided into four treatment types: 1. Negative control; 2. Positive control with MMS; 3. Treatments with the three forms of extracts (60, 25 and 4°C); and 4. Treatments with the extracts in different associations (simultaneous, pre-treatment, post-treatment and simultaneous after pre-incubation for 1 h) with MMS. None of the A. blazei extracts show genotoxic activity. In the comet assay no protecting effect was found. The results obtained in the MN test showed that the three forms of extracts used had protective activity, suggesting that the compound or active ingredients of A. blazei are always present in these extracts. The greater protective efficiency of the simultaneous treatment and simultaneous treatment with pre-incubation mixture with MMS suggests that the extracts have an antimutagenic action of the desmutagenic type. © 2002 Elsevier Science Ltd. All rights reserved.
dc.languageeng
dc.relationFood and Chemical Toxicology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAgaricus blazei
dc.subjectAnti-genotoxicity
dc.subjectCell culture
dc.subjectComet test
dc.subjectMethyl methanesulfonate
dc.subjectMicronucleus test
dc.subjectAgaricus blazei extract
dc.subjectantimutagenic agent
dc.subjectplant extract
dc.subjectunclassified drug
dc.subjectBrazil
dc.subjectcell culture
dc.subjectcell strain V 79
dc.subjectcomet assay
dc.subjectdrug effect
dc.subjectdrug isolation
dc.subjectgenotoxicity
dc.subjectin vitro study
dc.subjectincubation time
dc.subjectmammal
dc.subjectmicronucleus
dc.subjectreduction
dc.subjectAgaricus
dc.subjectAnimals
dc.subjectAntimutagenic Agents
dc.subjectComet Assay
dc.subjectCricetinae
dc.subjectCulture Media
dc.subjectDNA Damage
dc.subjectMicronucleus Tests
dc.subjectMutagens
dc.subjectTemperature
dc.subjectTumor Cells, Cultured
dc.subjectBasidiomycota
dc.subjectBovinae
dc.subjectFungi
dc.subjectMammalia
dc.titleAnti-genotoxic effect of aqueous extracts of sun mushroom (Agaricus blazei Murill lineage 99/26) in mammalian cells in vitro
dc.typeOtro


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