Differential gene expression in Trypanosoma cruzi populations susceptible and resistant to benznidazole

dc.creatorMurta, Silvane Maria Fonseca
dc.creatorRaad, Fernanda Barbosa Nogueira
dc.creatorSantos, Paula Fernandes dos
dc.creatorNunes, Fernanda Freire Campos
dc.creatorVolpe, Caroline Maria de Oliveira
dc.creatorLiarte, Daniel Barbosa
dc.creatorNirde, Philippe
dc.creatorProbst, Christian Macagnan
dc.creatorKrieger, Marco Aurelio
dc.creatorGoldenberg, Samuel
dc.creatorRomanha, Alvaro José
dc.date2022-02-09T19:08:13Z
dc.date2022-02-09T19:08:13Z
dc.date2008
dc.date.accessioned2023-09-26T22:55:56Z
dc.date.available2023-09-26T22:55:56Z
dc.identifierMURTA, Silvane Maria Fonseca et al. Differential gene expression in Trypanosoma cruzi populations susceptible and resistant to benznidazole. Acta Trop., v. 107, n.1, p. 59-65, 2008 .doi: 10.1016/j.actatropica.2008.04.011.
dc.identifier0001-706X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/51123
dc.identifier10.1016/j.actatropica.2008.04.011
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8884906
dc.descriptionCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO
dc.descriptionDifferential gene expression in three pairs of Trypanosoma cruzi populations or clones susceptible or resistant to benznidazole (BZ) was investigated by differential display (DD) and representation of differential expression (RDE). GenBank searches of 14 genes selected by DD showed that four sequences corresponded to different hypothetical proteins and the others were very similar to T. cruzi genes encoding mucin (TcMUC), dihydrolipoamide dehydrogenase (TcLipDH), the hexose transporter (TcHT), or a ribosomal protein. Sequence analysis was performed on 34 clones obtained by RDE; approximately half of these clones encoded 14 different hypothetical proteins and the other half encoded proteins involved with stress response, antioxidant defence, metabolism, transporter proteins, surface proteins, ribosomal proteins and others. The mRNA levels of eight T. cruzi genes obtained by RDE and Do were analysed by northern blotting to confirm the differential expression of these sequences. For six of the eight genes, TcLipDH, TcHT, TcFeSOD-A (iron superoxide dismutase-A), TcHSP70, TcHSP100 (heat shock protein) and Tc52 (thiol-transferase), mRNA levels in the drug-resistant T. cruzi population were at least twice those in the susceptible population. Further analysis of TcHSP70 showed that although the levels of TcHSP70 mRNA were four-fold higher in T. cruzi BZ-resistant population, no corresponding increase was observed in the levels of TcHSP70 protein expression. The results suggest that TcHSP70 is not directly associated with the T. cruzi drug resistance phenotype.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectTrypanosoma cruzi
dc.subjectdrug resistance
dc.subjectdifferential gene expression
dc.subjectdifferential display (DD)
dc.subjectr(RDE) representation of differential expression
dc.subject70 kDa heat shock protein (HSP70
dc.titleDifferential gene expression in Trypanosoma cruzi populations susceptible and resistant to benznidazole
dc.typeArticle


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