In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury

dc.creatorSouza, Elen Mello de
dc.creatorHenriques-Pons, Andrea
dc.creatorBailly, Christian
dc.creatorLansiaux, Amelie
dc.creatorAraujo-Jorge, Tania C.
dc.creatorSoeiro, Maria de Nazaré C.
dc.date2020-03-24T17:43:19Z
dc.date2020-03-24T17:43:19Z
dc.date2004
dc.date.accessioned2023-09-26T22:54:35Z
dc.date.available2023-09-26T22:54:35Z
dc.identifierSOUZA, Elen Mello de et al. In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 7, p. 697-701, Nov. 2004.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/40487
dc.identifier10.1590/s0074-02762004000700006
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8884639
dc.descriptionPrimary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectCreatina quinase
dc.subjectEnsaios in vitro
dc.subjectDanos cardíacos
dc.subjectCreatine kinase
dc.subjectIn vitro assays
dc.subjectCardiac damage
dc.titleIn vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury
dc.typeArticle


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