A tobacco cDNA reveals two different transcription patterns in vegetative and reproductive organs

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSilva, I. da
dc.creatorAngelo, P. C. S.
dc.creatorMolfetta, J. B.
dc.creatorFerraz, M. T.
dc.creatorSilva, L. L. P. da
dc.creatorGoldman, G. H.
dc.creatorGoldman, M. H. S.
dc.date2014-05-27T11:20:29Z
dc.date2016-10-25T18:17:55Z
dc.date2014-05-27T11:20:29Z
dc.date2016-10-25T18:17:55Z
dc.date2002-08-01
dc.date.accessioned2017-04-06T01:02:59Z
dc.date.available2017-04-06T01:02:59Z
dc.identifierBrazilian Journal of Medical and Biological Research, v. 35, n. 8, p. 861-868, 2002.
dc.identifier0100-879X
dc.identifierhttp://hdl.handle.net/11449/66949
dc.identifierhttp://acervodigital.unesp.br/handle/11449/66949
dc.identifier10.1590/S0100-879X2002000800001
dc.identifierS0100-879X2002000800001
dc.identifier2-s2.0-0036703817.pdf
dc.identifier2-s2.0-0036703817
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2002000800001
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/888452
dc.descriptionIn order to identify genes expressed in the pistil that may have a role in the reproduction process, we have established an expressed sequence tags project to randomly sequence clones from a Nicotiana tabacum stigma/style cDNA library. A cDNA clone (MTL-8) showing high sequence similarity to genes encoding glycine-rich RNA-binding proteins was chosen for further characterization. Based on the extensive identity of MTL-8 to the RGP-1a sequence of N. sylvestris, a primer was defined to extend the 5′ sequence of MTL-8 by RT-PCR from stigma/style RNAs. The amplification product was sequenced and it was confirmed that MTL-8 corresponds to an mRNA encoding a glycine-rich RNA-binding protein. Two transcripts of different sizes and expression patterns were identified when the MTL-8 cDNA insert was used as a probe in RNA blots. The largest is 1,100 nucleotides (nt) long and markedly predominant in ovaries. The smaller transcript, with 600 nt, is ubiquitous to the vegetative and reproductive organs analyzed (roots, stems, leaves, sepals, petals, stamens, stigmas/styles and ovaries). Plants submitted to stress (wounding, virus infection and ethylene treatment) presented an increased level of the 600-nt transcript in leaves, especially after tobacco necrosis virus infection. In contrast, the level of the 1,100-nt transcript seems to be unaffected by the stress conditions tested. Results of Southern blot experiments have suggested that MTL-8 is present in one or two copies in the tobacco genome. Our results suggest that the shorter transcript is related to stress while the larger one is a flower predominant and nonstress-inducible messenger.
dc.languageeng
dc.relationBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGlycine-rich protein
dc.subjectNicotiana tabacum
dc.subjectPistil expression
dc.subjectRNA-binding protein
dc.subjectStress conditions
dc.subjectVirus infection
dc.subjectcomplementary DNA
dc.subjectglycine
dc.subjectmessenger RNA
dc.subjectRNA binding protein
dc.subjectcontrolled study
dc.subjectdefective virus
dc.subjectDNA determination
dc.subjectDNA library
dc.subjectDNA probe
dc.subjectexpressed sequence tag
dc.subjectflower
dc.subjectgene expression
dc.subjectgene identification
dc.subjectgenetic transcription
dc.subjectgenome
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectNorthern blotting
dc.subjectpetal
dc.subjectpistil
dc.subjectpistil stigma
dc.subjectpistil style
dc.subjectplant disease
dc.subjectplant leaf
dc.subjectplant morphology
dc.subjectplant ovary
dc.subjectplant reproduction
dc.subjectplant root
dc.subjectplant stem
dc.subjectplant stress
dc.subjectreverse transcription polymerase chain reaction
dc.subjectreview
dc.subjectsequence homology
dc.subjectSouthern blotting
dc.subjectstamen
dc.subjecttobacco
dc.subjectFlowers
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation, Developmental
dc.subjectGene Library
dc.subjectMolecular Sequence Data
dc.subjectNucleic Acid Hybridization
dc.subjectPlant Proteins
dc.subjectReproduction
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA-Binding Proteins
dc.subjectSequence Alignment
dc.subjectSequence Analysis, Protein
dc.subjectSequence Analysis, RNA
dc.subjectTobacco
dc.subjectNicotiana sylvestris
dc.subjectTabacum
dc.subjectunidentified tobacco necrosis virus
dc.titleA tobacco cDNA reveals two different transcription patterns in vegetative and reproductive organs
dc.typeOtro


Este ítem pertenece a la siguiente institución