| dc.creator | dosSantos, Washington Luis Conrado | |
| dc.creator | Rahman, Jameel | |
| dc.creator | Klein, Nigel | |
| dc.creator | Male, David K | |
| dc.date | 2017-07-11T12:00:11Z | |
| dc.date | 2017-07-11T12:00:11Z | |
| dc.date | 1995 | |
| dc.date.accessioned | 2023-09-26T22:53:52Z | |
| dc.date.available | 2023-09-26T22:53:52Z | |
| dc.identifier | dosSANTOS, W. L. C. et al. Distribution and analysis of surface charge on brain endothelium in vitro and in situ. Acta Neuropathologica, v. 90, p. 305-311, 1995. | |
| dc.identifier | 0001-6322 | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/20034 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8884504 | |
| dc.description | dosSantos, Washington Luis Conrado “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | |
| dc.description | Brazilian Research Council (CNPq) and the Multiple Sclerosis Society of Great Britain | |
| dc.description | Vascular endothelial cells are associated with a
number of anionic molecules. These anions are important
in endothelial function, particularly in regulating permeability,
haemostasis and cellular traffic. To explore the nature
and distribution of anions on the brain endothelial
cell (BEC) surface, we have examined rat brain endothelium
in culture, and in situ. The anionic sites were probed
with cationic colloidal gold and cationised ferritin, and visualised
by light microscopy. Additionally we compared
the distribution of the anionic sites on BEC with that present
on other endothelial cell types in culture. The predominant
anion detected on BEC was heparan sulphate
(HS). This was distributed throughout the cell membrane,
but was most densely associated with intercellular junctions.
This pattern was distinct from the anionic locations
observed in endothelia from aorta and epididymal fat microvessels.
The distribution of anions was dependent on
the age of cultured cells, with only minimal levels of HS
seen at the periphery of younger cells. The nature and distribution
of negative charge was different in situ. Here,
sialic acid was the major surface anion, with only a small
contribution from HS. The significance of these findings
are discussed in relation to endothelial function in normal
tissue and in pathological conditions. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Springer Verlag | |
| dc.rights | open access | |
| dc.subject | Endotélio | |
| dc.subject | Carga negativa | |
| dc.subject | Glicosaminoglicanos | |
| dc.subject | Sulfato de Heparan | |
| dc.subject | Ácido siálico | |
| dc.subject | Endothelium | |
| dc.subject | Negative charge | |
| dc.subject | Glycosaminoglycans | |
| dc.subject | Heparan sulfate | |
| dc.subject | Sialic acid | |
| dc.title | Distribution and analysis of surface charge on brain endothelium in vitro and in situ | |
| dc.type | Article | |
